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Plasmid#112225PurposeExpression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.DepositorInsertSuper-folder GFP
UseZebrafish expressionTagsExpressionMutationPromoterbeta actin 2 (zebrafish)Available sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 884-1066 (aka Vt)
Plasmid#46176DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-LAMP1-mGL
Plasmid#222394PurposeTo express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.DepositorInsertLAMP1 and mGreenlantern (LAMP1 Human, Synthetic, Homo Sapiens)
UseAAVTagsLAMP1 and mGreenLanternExpressionMammalianMutationPromoterCAG or CAGIGAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 EGFP-3XNLS
Plasmid#58468PurposeExpresses a nuclear-targeted eGFP on a CMV promoterDepositorInsert3X NLS with 3X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-human Dynamin 1aa
Plasmid#22163DepositorInserthuman Dynamin 1aa (DNM1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationD744NPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-Halo
Plasmid#193328Purposemammalian transient expression - expression of Lifeact-HaloDepositorInsertLifeact
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB6 ZO1myc
Plasmid#30317DepositorInsertZonula Occludens-1 (TJP1 Human)
UseTagsmycExpressionMammalianMutationnonePromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
HA-BICD2(1-500)-FRB
Plasmid#174648PurposeRapalog-inducible coupling to dynein adaptor BICD2DepositorInsertHA-BICD2(1-500)-FRB
UseTagsFRB and HAExpressionMammalianMutationmmBICD2(aa1-500): Lys213GluPromoterChicken beta-actinAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmtb-t7-alpha-bungarotoxin
Plasmid#69542PurposeFor in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.DepositorInsertalpha-bungarotoxin
UseTol2, beta-actin promoter, sp6,t7 for zebrafishTagsExpressionMutationPromoterbeta-actin, sp6, t7Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mRuby3-IRES-Puro
Plasmid#155231Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mRuby sensor
UseLentiviralTagsmRuby3ExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1
Plasmid#12209DepositorInsertPak1 (PAK1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Lyn11-FRB
Plasmid#155228Purposemembrane anchor for FKBP-FRB systemDepositorInsertMembrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-PAK1 T423E
Plasmid#12208DepositorInsertPak1 (PAK1 Human)
UseTagsMycExpressionMammalianMutationActivated Pak1: T423EPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
Cherry-LacRep
Plasmid#18985DepositorInsertLacI
UseTagsAviTag, SV40 NLS, and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1 K299R
Plasmid#12210DepositorInsertPak1 (PAK1 Human)
UseTagsMycExpressionMammalianMutationCatalytically inactive Pak1: K299RPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAGG>nls-hCas9-nls
Plasmid#99138PurposeExpresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryosDepositorInserthumanized Cas9
UseCRISPRTagsExpressionMutationPromoterChicken beta actin (CAG)Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
C1-iRFP-iLid-CaaX
Plasmid#155229Purposemembrane markerDepositorInsertiRFP C-term tagged with the ilID motif and the CaaX sequence derived from Kras4b. Membrane control
UseTagsiRFP-iLidExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYC3.60-C1
Plasmid#67899PurposeYellow Cameleon 3.60 in a clontech-style backbone for expression in mammalian cellsDepositorInsertYC3.60
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-FKBP12-C1
Plasmid#67900PurposeExpresses a fusion of mCherry with FKBP12 in mammalian cellsDepositorInsertmCherry-FKBP12
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Plekhh1
Plasmid#187367PurposeExpresses human Plekhh1 labelled with EGFPDepositorInsertPlekhh1
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only