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Plasmid#210136Purposeknock out WAVE2 in mammalian cellsDepositorInsertActin-binding protein WASF2 (WASF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgWAVE2-1
Plasmid#210135Purposeknock out WAVE2 in mammalian cellsDepositorInsertActin-binding protein WASF2 (WASF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SPN_ARR Q37A/R38A/N52R (Shank3)
Plasmid#175256PurposeSPN-ARR fragment with structure opening N52R and actin binding site Q37A/R38A mutations in EGFP backbone for mammalian expressionDepositorInsertSPN-ARR fragment with Q37A/R38A/N52R mutations from SHANK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::HIS3
Plasmid#227596PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::HIS3
UseTags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::URA3
Plasmid#227595PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::URA3
UseTags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
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CFP-CaaX
Plasmid#155232Purposemembane markerDepositorInsertCFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
YFP-CaaX
Plasmid#155233Purposemembane markerDepositorInsertYFP C-term tagged with the CaaX sequence derived from Kras4b. Membrane control
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-CFP-Lyn11
Plasmid#155234Purposemembane markerDepositorInsertLyn11 membrane targeted CFP. Membrane control
UseTagsCFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTB
Plasmid#112225PurposeExpression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.DepositorInsertSuper-folder GFP
UseZebrafish expressionTagsExpressionMutationPromoterbeta actin 2 (zebrafish)Available sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 884-1066 (aka Vt)
Plasmid#46176DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-LAMP1-mGL
Plasmid#222394PurposeTo express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.DepositorInsertLAMP1 and mGreenlantern (LAMP1 Human, Homo Sapiens, Synthetic)
UseAAVTagsLAMP1 and mGreenLanternExpressionMammalianMutationPromoterCAG or CAGIGAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-MPAct-mRuby3-IRES-Puro
Plasmid#155231Purposelentiviral MPAct constructDepositorInsertLentiviral expression of MPAct-mRuby sensor
UseLentiviralTagsmRuby3ExpressionMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-Halo
Plasmid#193328Purposemammalian transient expression - expression of Lifeact-HaloDepositorInsertLifeact
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1
Plasmid#12209DepositorInsertPak1 (PAK1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 EGFP-3XNLS
Plasmid#58468PurposeExpresses a nuclear-targeted eGFP on a CMV promoterDepositorInsert3X NLS with 3X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCB6 ZO1myc
Plasmid#30317DepositorInsertZonula Occludens-1 (TJP1 Human)
UseTagsmycExpressionMammalianMutationnonePromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1 K299R
Plasmid#12210DepositorInsertPak1 (PAK1 Human)
UseTagsMycExpressionMammalianMutationCatalytically inactive Pak1: K299RPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEGFPN1-human Dynamin 1aa
Plasmid#22163DepositorInserthuman Dynamin 1aa (DNM1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationD744NPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Lyn11-FRB
Plasmid#155228Purposemembrane anchor for FKBP-FRB systemDepositorInsertMembrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only