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pJK501

Plasmid

#72246

Purpose

Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

Depositor

T. J. Kappock

Inserts

N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Use

Tags

Expression

Bacterial

Mutation

Gln206 to Arg and changes tryptophan-151 to pheny…

Promoter

T7 and endogenous

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pFUSC8

Plasmid

#50428

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 8.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC7

Plasmid

#50427

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 7.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC6

Plasmid

#50426

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 6.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC5

Plasmid

#50425

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 5.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC4

Plasmid

#50424

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 4.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC3

Plasmid

#50423

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 3.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC2

Plasmid

#50422

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 2.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pFUSC10

Plasmid

#50430

Purpose

Plasmid compatible with the Golden Gate TALEN cloning system. To clone TAL effectors 10.5 RVDs long.

Depositor

Andrew Scharenberg

Article

Boissel et al Nucleic Acids Res. 2013 Nov 26.

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pDL96

Plasmid

#43910

Depositor

Stephen Elledge

Article

Lee et al Mol Cell. 2008 Oct 10;32(1):70-80. doi:

Insert

DIF1 (DIF1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

AAEAAA

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pCL20C-ddIPpoI

Plasmid

#49053

Purpose

Expresses the restriction enzyme IPpoI under the control of Shield1

Depositor

Michael Kastan

Article

Goldstein et al Proc Natl Acad Sci U S A. 2013 Sep 30.

Insert

DD-HA-ER-IPpoI

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP

Plasmid

#62348

Purpose

An empty optimized gRNA expression vector

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

none

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pT1UciMP

Plasmid

#62215

Purpose

UAS transgenesis vector

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

UAS-E1b

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2+ EGFP-3NLS

Plasmid

#165400

Purpose

Expresses nuclear localized EGFP

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

Nuclear Localization Signal

Use

Avian, xenopus, zebrafish

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2z

Plasmid

#62214

Purpose

pCS2 with antifreeze protein terminator instead of sv40

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Mrna synthesis

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pT1QciMP

Plasmid

#62216

Purpose

QUAS transgenesis vector

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

QUAS-E1b

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pJK057

Plasmid

#75036

Purpose

Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase, N-terminal His6 (H6EcPurE1) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

Depositor

JoAnne Stubbe, T. J. Kappock

Inserts

N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase (purK Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

changes residues 2-7 to His (SSRNNP>HHHHHH)

Promoter

T7 and n/a

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET15b-H74A-PvuRts1I

Plasmid

#86894

Purpose

express H74A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Use

Tags

His

Expression

Bacterial

Mutation

W205A

Promoter

T7

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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p64-101

Plasmid

#25911

Depositor

Kenneth Marcu

Article

Stanton et al Nucleic Acids Res. 1982 Oct 11. 10(19):5

Insert

Ig germline gamma-1 switch region alpha

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
pET15b-E228A-PvuRts1I

Plasmid

#86896

Purpose

express E228A variant PvuRts1I in ER2566 strain

Depositor

Matthias Bochtler

Article

Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

Insert

PvuRts1I (pvuRts1I )

Use

Tags

His

Expression

Bacterial

Mutation

E228A

Promoter

T7

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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