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Plasmid#86447PurposepBAc transposon with transformation marker for Tribolium transgenesis; drives ECFP (blue fluorescent) expression in the eyes (much stronger than usual 3XP3); (Transformation plasmid)DepositorInsert6xP3-Tc-hsp-ECFP-SV40
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRetro-XT-PAK2-CFP(puro)-L107F
Plasmid#81180PurposeRetroviral vector for expression of kinase active PAK2 with a CFP fusionDepositorInsertp21 (RAC1) activated kinase 2 (PAK2 Human)
UseRetroviralTagsCFPExpressionMutationL107FPromoterP-tight TREAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G
Plasmid#18017DepositorInsertFLIPglu D154A Delta13V ECFP-W67G
UseTagsECFP-W67G and VenusExpressionMammalianMutationmglB-D154A; non-functional ECFPPromoterAvailable sinceFeb. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT2 TIA1 RRM-CFP-FRBx5
Plasmid#103783Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertTIA1 RRM-CFP-FRBx5
UseTagsCFPExpressionMammalianMutationPromoterSV40Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-Kan
Plasmid#44948PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with Kan selectionDepositorInsertyoPS-CFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPS-CFP2-SpHis5
Plasmid#44853PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible CFP2 with SpHis5 selectionDepositorInsertyoPS-CFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmCIT-CFPGgVcl 1-419 control 2 FRET
Plasmid#46283DepositorInsertECFP + Vcl 1-419 (VCL Chicken)
UseTagsmCitrineExpressionMammalianMutationaa 1-419PromoterCMVAvailable sinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
V957 pLPS-3' ECFP-mito - Acceptor
Plasmid#11699DepositorTypeEmpty backboneUseCre/Lox; Acceptor vectorTagsECFP-mitoExpressionMammalianMutationPromoterAvailable sinceMay 26, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBEST-PLlacO-1-UTR1-deCFP-T500
Plasmid#45777DepositorInsertdeCFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
LA201: pMVP (L3-L2) P2A-eCFP + WPRE
Plasmid#121776PurposepMVP L3-L2 entry plasmid, contains eCFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eCFP linked by P2A to gene of interest.DepositorInsertP2A-eCFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-Flag-mIL-17RA/CFP.Hygro
Plasmid#46861Purposeencodes Flag-tagged mouse IL-17RA fused to Cyan Fl. ProteinDepositorInsertIL-17RA-CFP
UseTagsFlag/IL-3 signal sequence and fused to CFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-sgRNA-M9-IRES-CFP
Plasmid#114731PurposesgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorInsertsgRNA M9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
CFP(1-158)-gamma-1 in pcDNAI/Amp
Plasmid#55615PurposeAn amino-terminal CFP fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCFP(1-158)-gamma-1 (GNGT1 Aequorea victoria, Bovine)
UseTagsCFP(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-1 was amplified by PCR, which added a BamH…PromoterCMVAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFP(1-158)-gamma-2 in pcDNAI/Amp
Plasmid#55616PurposeAn amino-terminal CFP fragment was fused to Ggamma-2. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCFP (1-158)-gamma-2 (Gng2 Aequorea victoria, Mouse)
UseTagsCFP(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-2 was amplified by PCR, which added a BamH…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-577-CFP-578CT
Plasmid#41682DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
UseTagsCFPExpressionMammalianMutation“Monomerizing” CFP A206K mutation; CFP inserted d…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b/ECFP-GgVcl 884-1066 (Vt)
Plasmid#46180DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsECFP and HisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available sinceJuly 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJRL2 PHO5(TATA-C2)pr ECFP (EB#1562)
Plasmid#14844DepositorInsertPHO5 promoter TATA-C2 (PHO5 Budding Yeast)
UseTagsECFPExpressionYeastMutationTATA-C2. TATA box mutated from TATATAAG to TCTA…PromoterAvailable sinceMay 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
elavl3:hGCGR-TEVcs-QF;he1.1:CFP
Plasmid#218852PurposeD. rerio elavl3 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-TangoDepositorInserthGCGR (GCGR Human)
UseTagsQF fusion proteinExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP
Plasmid#218857PurposeXla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.DepositorInserthGCGR (GCGR Human)
UseTagsQF fusion proteinExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:NLS-mApple-he1.1:CFP
Plasmid#184814PurposeUsed to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic lineDepositorArticleInsertQUAS:NLS-mApple-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsNLS-mAppleExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only