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Plasmid#197590PurposeLentiviral transfer vector for the neuronal expression of membrane-bound TagBFP2 (good visualization of neuronal processes)DepositorInsertmTagBFP2-CAAX
UseLentiviralTagsCAAXExpressionMutationPromoterhSynIAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomTagBFP-SpHis5
Plasmid#44838PurposeYeast tagging vector to create gene fusion with yeast optimized mTagBFP with SpHis5 selectionDepositorInsertyomTagBFP
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion T2A-H2B-TagBFP
Plasmid#113086PurposeGeneral donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertT2A-H2B-TagBFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTag-BFP-C-h-Rab5a-c-Myc
Plasmid#79801PurposeExpress TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).DepositorInsertRab5a (RAB5A Human)
UseTagsTagBFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP
Plasmid#102797PurposeRetrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroRDepositorInsertssgRNA
EGFP
UseCRISPR and RetroviralTagsExpressionMammalianMutationH231LPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR EGFR-SLP76-TagBFP
Plasmid#188630PurposeHuman EGFR labeled with SLP76 for monitoring with SLP76/VISH2 pYtag biosensorDepositorInsertEGFR (EGFR Human, Synthetic)
UseLentiviralTagsSLP76-TagBFPExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRepair-MPP8_mAID-P2A-BFP
Plasmid#175036PurposeHomology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9DepositorInsertmAID-P2A-BFP
UseHdr donor templateTagsExpressionMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3GS-->sGFP-P2A-BFP
Plasmid#162234PurposeLentiviral vector for the expression of TRE3GS-->sGFP-P2A-BFP in mammalian cellsDepositorInsertEGFP and BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-FLPo
Plasmid#124834PurposeMonomeric TagBFP2 and FLP recombinaseDepositorInsertmTagBFP2-T2A-FLPo
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
SBFP2-anti-AlfaTag nanobody
Plasmid#171826PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertSBFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mTagBFP2 PuroR
Plasmid#101893PurposeLentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmTagBFP2ExpressionMutationPromoterCMV immediate earlyAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP
Plasmid#67991PurposeCRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPREDepositorInsertU6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AKAP-mtagBFP2-UBC6
Plasmid#105007PurposeMammalian Expression of mtagBFP2DepositorInsertmtagBFP2
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pEF1a-EBFP-tTAn
Plasmid#172122PurposeConstitutive expression of EBFP and rtTA-InteinNDepositorInsertEBFP, tTAn-InteinN
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W
Plasmid#105034PurposeLentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFPDepositorInsertPtpn23 (Ptpn23 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-mTagBFP2-T2A-iCre (v2)
Plasmid#125822PurposeMonomeric TagBFP2 and iCre recombinase (version 2)DepositorInsertmTagBFP2-T2A-iCre
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV-U6gRNA-EF(BbsI)-PGKpuro2ABFP
Plasmid#62348PurposeAn empty optimized gRNA expression vectorDepositorInsertnone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pegRNA-GFP>BFP_Y66H-NGG
Plasmid#185480PurposepegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene.DepositorInsertGFP>BFP_Y66H-pegRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL2-mGold-P2A-EBFP2
Plasmid#157998PurposeCo-expression of mGold and EBFP2 in mammalian cells.DepositorInsertmGold-P2A-EBFP2
UseTagsExpressionMammalianMutationmGold is mVenus with L46F;T63S mutationsPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; HE1A:BFP -0
Plasmid#180007PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; HE1A: BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only