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GUT BuGZ delta ZF1, delta ZF2

Plasmid

#84027

Purpose

Expression of GFP tagged mutant BuGZ in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

delta 13-58; Q394R

Promoter

UCOE / SFFV

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pR2Q306c

Plasmid

#101027

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

Glutamate receptor subunit (Gria2 Rat)

Use

Tags

fluorescent protein

Expression

Mammalian

Mutation

tagged with fluorescent protein

Promoter

CMV

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shCdk1

Plasmid

#160953

Purpose

Cdk1 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Cdk1 shRNA (Cdk1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pST1374-H840A-GCN4

Plasmid

#113027

Purpose

Expresses H840A-GCN4 in mammalian cells

Depositor

Xingxu Huang

Article

Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

Insert

H840A-GCN4 (GCN4 S. pyogenes, Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

D839A, H840A, N863A

Promoter

CMV

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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BMR1_04g05295-bio-His

Plasmid

#108027

Purpose

Expresses enzymatically monobiotinylated full-length BMR1_04g05295 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

Insert

BMR1_04g05295

Use

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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P789_pY026_EnAsCpf1_CLYBL_T1_catRNA

Plasmid

#202760

Purpose

Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the CLYBL safe harbor site

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

huAsCpf1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMX-IG-ATG3 (K62D/K64D)

Plasmid

#212031

Purpose

Expresses untagged ATG3 (K62D/K64D) in mammalian cells

Depositor

Michael Lazarou

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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SUMO-Twinkle 43-372

Plasmid

#205054

Purpose

TWNK protein expression

Depositor

Smita Patel

Article

Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

Insert

TWNK (TWNK Human)

Use

Tags

Expression

Bacterial

Mutation

Lacks Mitochondrial localization signal and C ter…

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.23-Gja1prom

Plasmid

#188114

Purpose

Expresses luciferase under control of Gja1 promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

Insert

Gja1 promoter (Gja1 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Gja1 endogenous promoter up to gene start codon

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2C

Plasmid

#202736

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2C (PIP4K2C, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2CD280K

Plasmid

#202737

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2C (D278K) (PIP4K2C, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2BD278K

Plasmid

#202735

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A (3-108):[GGSA]4GG:PIP4K2B (D280K) (PIP4K2B, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2A

Plasmid

#202732

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2A (PIP4K2A, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2AD273K

Plasmid

#202733

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2A (D266K) (PIP4K2A, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hASCL1 gRNA_1-MS2-Puro

Plasmid

#192670

Purpose

Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human ASCL1 activating gRNA #1 (ASCL1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL1RN gRNA_1-MS2-Puro

Plasmid

#192677

Purpose

Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNA #1 (IL1RN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRps9-WT-IRES-GFP-HygR

Plasmid

#190271

Purpose

Express RPS9 WT mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.

Depositor

Erik Böttger

Article

Brilkova et al Cell Rep. 2022 Sep 27;40(13):111433. doi

Insert

ribosomal protein RPS9

Use

Tags

IRES-eGFP

Expression

Mammalian

Mutation

Promoter

CAGGS-CMV

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pRps9-D95N-IRES-GFP-HygR

Plasmid

#190272

Purpose

Express RPS9 D95N-mutant mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.

Depositor

Erik Böttger

Article

Brilkova et al Cell Rep. 2022 Sep 27;40(13):111433. doi

Insert

ribosomal protein RPS9

Use

Tags

IRES-eGFP

Expression

Mammalian

Mutation

D95N

Promoter

CAGGS-CMV

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 D-4

Plasmid

#184016

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 651 to 665.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-4 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Prom1 D-6

Plasmid

#184018

Purpose

Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 631 to 645.

Depositor

Peter Stoilov

Article

Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

Insert

Prom1 SV8(-Ex19a) D-6 (Prom1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of exon 19a (amino acids 696-701), and d…

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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