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Plasmid#121418PurposepUC19_BsaI-dCas9-BsaIDepositorInsertdcas9
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR
Plasmid#113252PurposepUC19 backbone with E. coli CRISPR arrayDepositorInsertE. coli CRISPR array
UseIntegration substrateTagsExpressionMutationPromoterNAAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW091
Plasmid#113253PurposepUC19 backbone with S. pyogenes CRISPR arrayDepositorInsertS. pyogenes CRISPR array
UseIntegration substrateTagsExpressionMutationPromoterNAAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
NK601
Plasmid#109070PurposeParental vector for making S. rosetta expression vectorsDepositorInsertPTSG_03784
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
NK586
Plasmid#109069PurposeParental vector for making S. rosetta expression vectorsDepositorInsertPTSG_05705
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
NK583
Plasmid#109072PurposeParental vector for making S. rosetta expression vectorsDepositorInsertPTSG_10057
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
NK600
Plasmid#109068PurposeParental vector for making S. rosetta expression vectorsDepositorInsertPTSG_02256
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSAP
Plasmid#206429PurposeA vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT10
Plasmid#200989PurposepL0V-Lv1-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApRDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP4
Plasmid#206850PurposeA plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPt_Cp
Plasmid#206855PurposeA plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. The marker URA3 was integrated into the genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCHAP3_Sap
Plasmid#206849PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCVD432
Plasmid#63755PurposeProbe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.DepositorInsertEAggEC
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP8
Plasmid#206857PurposeA plasmid containing a 11,944 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP7
Plasmid#206853PurposeA plasmid containing a 13,748 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPt_Sap
Plasmid#206856PurposeA plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. It contains a single SapI site located in the URA3 marker, which was integrated into the genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP6
Plasmid#206852PurposeA plasmid containing a 15,671 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP5
Plasmid#206851PurposeA plasmid containing a 17,639 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP3_URA
Plasmid#206848PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was integrated into the chloroplast fragment, which is flanked by SapI sites.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rp51a Hyperstabilized 5'SS
Plasmid#100988PurposepUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGTDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutation5'ss mut ATG/GTATGT -> AAG/GTAAGTPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-NoBS-NoPseudoBS
Plasmid#100986PurposeRP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationBS mutated to GTTAGTG, pseudo BS mutated to GTTTG…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1119 (pDonor)
Plasmid#160732PurposeMini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSelAct-KO
Plasmid#174374PurposePlasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatibleDepositorTypeEmpty backboneUseVector for 2-step knockout by homologous recombin…TagsExpressionMutationPromoterN/AAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
CFP_Rad_pcDNA4_HisMaxC
Plasmid#41654DepositorInsertRad, GTP binding proteins (RGK) (Rrad Mouse)
UseTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSelAct-Express
Plasmid#174375PurposePlasmid for integrative expression, pUC19 backbone, Apr resistant casette, pCMP1::eGFP expression cassette, gateway compatibleDepositorTypeEmpty backboneUseExpression vector for targeted integrationTagsExpressionMutationPromoterpCMP1Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT440
Plasmid#8609DepositorInsertbarnase, barstar
UseTagsas pMT416ExpressionBacterialMutationV36 codon of barnase of pMT416 replaced by polyli…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHB1
Plasmid#204668PurposepUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructsDepositorInsertFRT-erm-FRT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGT5439
Plasmid#192351PurposeL0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stopDepositorInsertTTCG_Stop-tOCS_CGCT
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGT9403
Plasmid#192352PurposeL0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stopDepositorInsertTTCG_Stop-tNOS_CGCT
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGT10446
Plasmid#192353PurposeL0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stopDepositorInsertTTCG_Stop-t35S_CGCT
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-No5'ss-NoBS-NoPseudoBS
Plasmid#100987PurposeRP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutation5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5343_UC57
Plasmid#126645Purposeenvironmental plasmid p5343 cloned into pUC57DepositorInsertEnvironmental plasmid p5343
UseSynthetic BiologyTagsExpressionMutationPromoterNAAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCVD434
Plasmid#63757PurposeEPEC eae probe. 1.1 kb Sa1I/StuI fragment of E2348/69 (from gene bank cosmid II E1) cloned into Sa1I/SmaI site of pUC18.DepositorInserteae probe
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pSL1749 (pDonor, R*MmeI)
Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAT.015
Plasmid#119558PurposeLevel T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP6534
Plasmid#128735PurposeGateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
CFP_Rem_pcDNA4_HisMaxC
Plasmid#41651DepositorInsertRem, GTP binding proteins (RGK) (Rem1 Mouse)
UseTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCPP6533
Plasmid#128734PurposeGateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal Cya tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAT.334
Plasmid#119556PurposeLevel T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-PH-TAPP1
Plasmid#161985PurposeExpresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)DepositorInsertPH-TAPP1 (PLEKHA1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CFP_Gem_pcDNA4_HisMaxC
Plasmid#41653DepositorInsertGem, GTP binding proteins (RGK) (GEM Human)
UseTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAGT8162 (AATG_dttLbCas12a-i_TTCG)
Plasmid#192350PurposeL0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expressionDepositorInsertAATG_dttLbCas12a-i_TTCG
UseCRISPRTagsExpressionMutationD156R; E925QPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV10
Plasmid#63213PurposepUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
GFP-PIK3C2A
Plasmid#161988PurposeMammalian expression vector containing PIK3C2A tagged with GFPDepositorInsertPIK3C2A (PIK3C2A Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGT8163 (AATG_ttLbCas12a-i_TTCG)
Plasmid#192453Purposeintronized ttLbCas12a for in planta expressionDepositorInserttemperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
UseCRISPR and Synthetic Biology; Moclo compatible le…TagsExpressionMutationtemperature-tolerant mutation (D156R)PromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only