pCVD432
(Plasmid
#63755)
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PurposeProbe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 63755 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC19
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEAggEC
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GenBank IDX81423.1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site PstI (unknown if destroyed)
- 5′ sequencing primer pBRrevBam (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DNA probe for the identification of enteroaggregative E. coli
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCVD432 was a gift from James Kaper (Addgene plasmid # 63755 ; http://n2t.net/addgene:63755 ; RRID:Addgene_63755) -
For your References section:
A sensitive and specific DNA probe to identify enteroaggregative Escherichia coli, a recently discovered diarrheal pathogen. Baudry B, Savarino SJ, Vial P, Kaper JB, Levine MM. J Infect Dis. 1990 Jun;161(6):1249-51. PubMed 2189007