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Showing: 1351 - 1400 of 15599 results

  1. pLV-SFFV-mCherry-WPRE-UbC-Emerald

    Plasmid
    #211797
    Purpose
    Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Inserts
    mCherry
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211797-plasmid-map-sequence-id-418065

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  2. pDONR221-deltaUBA-short-UBASH3A-Ctag

    Plasmid
    #195397
    Purpose
    Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for C-terminal tag)
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    UBASH3A mutant lacking the UBA domain
    Promoter
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195397-plasmid-map-sequence-id-388659

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  3. pDONR221-deltaUBA-short-UBASH3A-Ntag

    Plasmid
    #195398
    Purpose
    Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for N-terminal tag)
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Gateway donor vector
    Tags
    Expression
    Mutation
    UBASH3A mutant lacking the UBA domain
    Promoter
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    195398-plasmid-map-sequence-id-386315

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  4. pGEX-6P-1-PDE-short-UBASH3A

    Plasmid
    #195403
    Purpose
    Bacterial expression of a putative PDE domain of human short-form UBASH3A with GST tag
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    PDE domain of UBASH3A
    Promoter
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    195403-plasmid-map-sequence-id-386313

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  5. deltaUBA-short-UBASH3A-V5-6xHis

    Plasmid
    #195404
    Purpose
    Mammalian expression of human short-form UBASH3A lacking the UBA domain with V5-6xHis tag
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Tags
    V5-6xHis
    Expression
    Mammalian
    Mutation
    UBASH3A mutant lacking the UBA domain
    Promoter
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    195404-plasmid-map-sequence-id-386311

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  8. BPV00500 pET21GG2 His(6)-TF-BECN(1-450)

    Plasmid
    #136684
    Purpose
    Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)
    Depositor
    Colin Garvie
    Article
    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66
    Insert
    Beclin-1 (BECN1 Human)
    Use
    Tags
    Trigger Factor
    Expression
    Bacterial
    Mutation
    DNA optimized for E. coli expression
    Promoter
    T7
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136684-plasmid-map-sequence-id-272124

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  10. pT3-EF1aH-PIK3CA H1047R

    Plasmid
    #180031
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.
    Depositor
    Xin Chen
    Article
    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv
    Insert
    PIK3CA H1047R (PIK3CA Human)
    Use
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    H1047R
    Promoter
    EF1a
    Available since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180031-plasmid-map-sequence-id-357864

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  20. pLV-SFFV-MCS-WPRE-UbC-mCherry

    Plasmid
    #211799
    Purpose
    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Inserts
    multiple cloning site
    mCherry
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211799-plasmid-map-sequence-id-418073

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  21. pCWXPG-UBI-Glis3M412

    Plasmid
    #114301
    Purpose
    Ubiquitous expression of a constitutively active form of the Glis3 transcription factor
    Depositor
    Patrick Salmon
    Insert
    Glis3 (Glis3 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    N-terminal deletion up to Methionine 412
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114301-plasmid-map-sequence-id-218683

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  22. EGFP-hPMCA4bct120

    Plasmid
    #47593
    Depositor
    Emanuel Strehler
    Insert
    PMCA4bct120 (ATP2B4 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    120 C terminal AA deleted (constitutively active …
    Promoter
    CMV
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. pLV-SFFV-mCherry-WPRE-UbC-MCS

    Plasmid
    #211801
    Purpose
    Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Inserts
    mCherry
    multiple cloning site
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211801-plasmid-map-sequence-id-418105

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  25. pLV-SFFV-MCS-WPRE-UbC-Emerald

    Plasmid
    #211796
    Purpose
    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Inserts
    multiple cloning site
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211796-plasmid-map-sequence-id-418059

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  26. psubCMV-mVEGF-D-dNdC-WPRE

    Plasmid
    #119228
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has native signal peptide to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    Vegfd-dN-dC (Vegfd Mouse)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    119228-plasmid-map-sequence-id-231686

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  27. pOET5 rota-VLP

    Plasmid
    #218147
    Purpose
    Forms rotavirus-like particles in insect cells by expression of VP6 (GenBank ID: ACL93331.1).
    Depositor
    Minna Hankaniemi
    Insert
    Rotavirus VP6
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218147-plasmid-map-sequence-id-433323

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  28. pLV-SFFV-Emerald-WPRE-UbC-MCS

    Plasmid
    #211800
    Purpose
    Lentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Inserts
    Emerald
    multiple cloning site
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211800-plasmid-map-sequence-id-418102

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  29. pLV-SFFV-MCS-WPRE-UbC-MCS

    Plasmid
    #211798
    Purpose
    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and another multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Inserts
    multiple cloning site
    multiple cloning site
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211798-plasmid-map-sequence-id-418070

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  30. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87381-plasmid-map-sequence-id-212242

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  31. BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

    Plasmid
    #136688
    Purpose
    Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.
    Depositor
    Colin Garvie
    Article
    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66
    Insert
    Beclin-1 (BECN1 Human)
    Use
    Tags
    Trigger Factor
    Expression
    Bacterial
    Mutation
    DNA optimized for E. coli expression w/ three mut…
    Promoter
    T7
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136688-plasmid-map-sequence-id-272126

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  35. ACSM2B

    Plasmid
    #39167
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACSM2B (ACSM2B Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Expression
    Mutation
    Actually the mitochondrial form, GI:58082049, wit…
    Promoter
    polyhedrin
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  36. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

    Plasmid
    #119229
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    IL3SP-Vegfd-dNdC (Vegfd Mouse)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119229-plasmid-map-sequence-id-239316

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  37. pAAV2-CAGIG-WGA-mCherry

    Plasmid
    #224119
    Purpose
    To express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.
    Depositor
    Pantelis Tsoulfas
    Insert
    WGA-mCherry
    Use
    AAV
    Tags
    WGA and mCherry
    Expression
    Mammalian
    Mutation
    All mutations related to the WGA in this construc…
    Promoter
    CAGIG
    Available since
    Sept. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    224119-plasmid-map-sequence-id-446520

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  38. pMSCVpuro-Mito-DsRed1

    Plasmid
    #87379
    Purpose
    Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).
    Depositor
    Björn Stork
    Article
    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do
    Insert
    TXN2 mitochondrial transit peptide (TXN2 Human)
    Use
    Retroviral
    Tags
    DsRed1
    Expression
    Mammalian
    Mutation
    First 60 aa only
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    87379-plasmid-map-sequence-id-212256

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  40. pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

    Plasmid
    #217014
    Purpose
    Expression of CDK2-7 chimera in E.coli
    Depositor
    Manjeet Mukherjee
    Article
    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018
    Insert
    CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) (CDK7, CDK2 Human)
    Use
    Tags
    C3 protease cleavable GST tag
    Expression
    Bacterial
    Mutation
    CDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217014-plasmid-map-sequence-id-431050

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Showing: 1351 - 1400 of 15599 results