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Plasmid#209672PurposeExpresses CRY2 fused with Split-TurboID-C and mCherry in mammalian cellsDepositorInsertCRY2 (CRY2 Mustard Weed)
UseLentiviralTagsSplit-TurboID-C and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CibN
Plasmid#209671PurposeExpresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cellsDepositorInsertCIB1 (CIB1 Mustard Weed)
UseTagsSplit-TurboID-N and pmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0001
Plasmid#179834Purposebinary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoterDepositorInserteYGFPuv
UseBinary vector for plant transformationTagsExpressionPlantMutationPromoter35SAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti X1 Puro empty (w610-1)
Plasmid#20953Purpose3rd gen lentiviral empty control vector, PuroDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAuroraA-GFP-AURKA
Plasmid#99877PurposeExpression of GFP-AURKA in mammalian cellsDepositorInsertAURKA (AURKA Human)
UseTagsGFPExpressionMammalianMutationPromoterAURKA minimalAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
GPRC5D-Tango
Plasmid#66385PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPRC5D (GPRC5D Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES
Plasmid#200406PurposeExpresses a compact, engineered version of ADAR2 constitutively in mammalian cellsDepositorInsertMCP-ADAR2dd(E488Q)-NES
UseSynthetic BiologyTagsExpressionMammalianMutationE488Q (hyperactive)PromoterCMVAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pThy1-Brainbow3.2
Plasmid#45179DepositorInsertpThy1-Brainbow3.2
UseLinearized for transgenic mouse injectionTagsExpressionMutationPromoterThy1Available sinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
MOS
Plasmid#64120PurposeA modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genesDepositorUseTagsHA and MycExpressionMammalianMutationPromoterSFFVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRE5
Plasmid#62377PurposeFluorescent reporter to quantify the ratio of two alternative splicing patterns.DepositorInsertdsRed and GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 blast
Plasmid#104997PurposeThis lentiviral construct delivers hSpCas9 and blasticidin S resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Akaluc-N1
Plasmid#120371PurposeAkaluc luciferase mammalian expression in -N1 backboneDepositorInsertAkaluc (human optimized)
UseTagsExpressionMammalianMutationHuman codon optimizedPromoterCMVAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHUE411
Plasmid#62203PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterOsU3 and UbiAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
p(HA)HIF1alpha (P402A,P564A)
Plasmid#52636Purposemammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutationDepositorInsertHIF1alpha P402A, P564A (HIF1A Human)
UseTagsHAExpressionMammalianMutationP402A, P564APromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
EGFR (del3) L747-E749del, A750P
Plasmid#11015PurposeRetroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cellsDepositorInsertEGFR (del3) (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationL747-E749 deleted, A750PPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGreen_dualluc_3'UTR_sensor
Plasmid#55206PurposeDual-luciferase reporter for microRNA targeting in plantsDepositorInsertsRenilla luciferase
Firefly luciferase
UseLuciferase; Plant transient expressionTagsExpressionMutationPromoterAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NR2A
Plasmid#17924DepositorInsertNMDAR2A (Grin2a Rat)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQE30 PTBP1 isoform1
Plasmid#108591PurposeRecombinant His tagged protein productionDepositorInsertPTBP1 isoform 1 (PTBP1 Human)
UseTags6xHisExpressionBacterialMutationisoform 1 (isoform c)PromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_H2A_C1
Plasmid#85051PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsertH2A (H2AC16 Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRE_GFP-GluR1
Plasmid#34857DepositorInsertGluR1 (Gria1 Mouse)
UseMouse TargetingTagsGFPExpressionMammalianMutationPromoterTREAvailable sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI-SEP-NRI
Plasmid#23999DepositorInsertNR1 (Grin1 Rat)
UseTagsNR1 signal sequence (aa1-20) and SEP (supereclipt…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W
Plasmid#67982PurposeCas9 activity reporter with mCherry and GFPDepositorInsertU6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRY-Gal∆DD (B1013)
Plasmid#92035PurposeExpresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRESDepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationFusion of plant CRY2 to Gal4 binding domain (AA1-…PromoterAvailable sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJRD215
Plasmid#167555PurposeBackbone plasmid to pRMA154DepositorTypeEmpty backboneUseBroad host range mobilisableTagsExpressionMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Phi3-AURKB-PGK-mCherry
Plasmid#153316PurposeExpresses AURKB and mCherry in mammalian cells.DepositorInsertAURKB (AURKB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pATT-Dest
Plasmid#79770PurposeStaging plasmid for use in DIP transpositionDepositorTypeEmpty backboneUseSynthetic Biology; Staging plasmid for transposit…TagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGreen_GUS_competitor
Plasmid#55208PurposeGUS-based microRNA target competitor used for testing function as target mimicryDepositorInsertbeta-glucuronidase
UsePlant expressionTagsExpressionMutationPromoterAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-L2
Plasmid#127352Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir
Plasmid#90004PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]DepositorInsertsFKBP-linker-Myc-NBirA*
CBirA*-linker-FRB
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_LaG17-mCherry-HOTag3
Plasmid#190024PurposeTransiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.DepositorInsertLaG17 CluMPS
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_NUP98_KDM5A
Plasmid#205872PurposeExpress mEGFP-tagged fusion protein, NUP98_KDM5A from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 sgKEAP1-3
Plasmid#186837Purposeknock out KEAP1 in mammalian cellsDepositorInsertKeap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN1-Lck-GCaMP2
Plasmid#24794DepositorInsertLck-GCaMP2
UseTagsHis and XpressExpressionMammalianMutationPlasmid contains N-terminal 26 amino acid membran…PromoterAvailable sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-DUX4-CA
Plasmid#99281PurposeAll-in-one lentivector with tet-inducible DUX4 (codon sequences altered)DepositorInsertDUX4 (codon altered) (DUX4 Human)
UseLentiviralTagsExpressionMammalianMutationcodon alteredPromoterTREAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-p300_Blast
Plasmid#192653Purpose3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance markerDepositorInsertdCas9-p300
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A; H840A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
plRL19
Plasmid#163634PurposeL5 Integrase delivery plasmid to allow integration of L5 attP only plasmids (e.g. plRL61, ID #163633).DepositorInsertL5 Integrase
UseTagsExpressionBacterialMutationPromoterMycobacterial Optimized Promoter (MOP) and Tet-OnAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PLE-IRES-mCherry
Plasmid#45766DepositorInsertesterase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-YAP shRNA2
Plasmid#166485PurposeHuman YAP RNAiDepositorInsertshRNA for human YAP (YAP1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(+)-NEMOf
Plasmid#189932PurposeMammalian expression of calcium sensor with ultra-high dynamics and sensitivityDepositorInsertNEMOf
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
epB_UbC_CymRV5-nls-Halox2_DEx4
Plasmid#119907PurposeePiggyBac mammalian expression plasmid for CymR-Halo fusionDepositorInsertCymRV5-nls-Halox2
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
plRL61
Plasmid#163633PurposeOptimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 sgKEAP1-2
Plasmid#186459Purposeknock out KEAP1 in mammalian cellsDepositorInsertKeap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCag FlpO-2A-Cre EV
Plasmid#129419Purposeepisomal expression of FlpO and Cre recombinasesDepositorInsertFlpO-2A-Cre
UseCre/Lox and Unspecified; Episomal expression vect…TagsExpressionMammalianMutationPromoterCMV/Chick β-actin (CAG)Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPC-N FLAG
Plasmid#12521DepositorInsertN FLAG
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAV-flex-plap
Plasmid#80422Purposetracing neuronal projectionDepositorInsertplap (ALPP Human)
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXL001-TQM IL13 CAR
Plasmid#154054PurposeLentiviral expression of TQM IL13 CAR for CAR-T therapyDepositorInsertTQM IL13 CAR
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMVlux-cp157Venus
Plasmid#197584PurposeBRET lux luciferase with cp157Venus for mammalian expressionDepositorInserthuman codon-optimized Lux-cp157Venus
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5M-TACE
Plasmid#31714DepositorInsertTACE (ADAM17 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
plRL58
Plasmid#166886PurposeOptimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1a-Bmi1
Plasmid#31783DepositorInsertBmi1 (BMI1 Human)
UseTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits only