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Plasmid#133506PurposeExpression of eGFP tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
YPet-Lysosomes-20
Plasmid#56636PurposeLocalization: Lysosome Membrane, Excitation: 513, Emission: 527DepositorInsertLAMP1
UseTagsYPetExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
Hs.NRAS G13D
Plasmid#83177PurposeGateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G13D mutationDepositorInsertNRAS (NRAS Human)
UseGateway entry cloneTagsExpressionMutationG13DPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-ERBB2-S310F
Plasmid#116349PurposeLentiviral expression of ERBB2 S310FDepositorInsertERBB2 (ERBB2 Human)
UseLentiviralTagsExpressionMutationS310FPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF1426
Plasmid#143270PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXK2 (FOXK2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CKM
Plasmid#23866DepositorInsertCKM (CKM Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC19/PLIN2 GFP-P2A-BFP
Plasmid#202195Purposeknock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2DepositorInsertPLIN2 (PLIN2 Human)
UseCloning vectorTagsGFP-P2A-BFP knock-inExpressionMutationPromoterlacAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
TPP1-bio-His
Plasmid#52090PurposeExpresses full-length Tripeptidyl-peptidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTPP1 (TPP1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB038a
Plasmid#99211PurposeAccessory plasmid for PACE of PylRS variantsDepositorInsertPpsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
UseTagsExpressionBacterialMutationamber codons at positions 29 and 184 of gIIIPromoterPpsp and PproKAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB038
Plasmid#99210PurposeAccessory plasmid for PACE of PylRS variantsDepositorInsertPpsp [SD8] gIII(P29*), luxAB; PproK pylT
UseTagsExpressionBacterialMutationamber codons at position 29 gIIIPromoterPpsp and PproKAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB038b
Plasmid#99212PurposeAccessory plasmid for PACE of PylRS variantsDepositorInsertPpsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
UseTagsExpressionBacterialMutationamber codons at positions 29, 83, and 184 of gIIIPromoterPpsp and PproKAvailable sinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1-TelR15-mCherry
Plasmid#49647PurposeTALERed-Telomere for In Vitro TranslationDepositorInsertTelR15
UseIn vitro translationTagsmCherryExpressionMutationPromoterT7Available sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pAV0785
Plasmid#133507PurposeExpression of mCherry tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-mCherry-pcn1-terminator(nmt1)-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMRE-Tn5-133
Plasmid#118519PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-131
Plasmid#118517PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRFP.mIKKε
Plasmid#74414PurposeExpresses mouse RFP-IKKε in mammalian cellsDepositorInsertmouse IKKε (Ikbke Mouse)
UseTagsRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-UN-Cas9
Plasmid#135011PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9DepositorInserthumanized S. pyogenes Cas9
UseTags126aa domain from HSV-1 UL12 fused to the N-termi…ExpressionMammalianMutation126aa domain from HSV-1 UL12 fused to the N-termi…PromoterCBhAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pExp-mCherry
Plasmid#112569PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-mCherry and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCINEO-3xFlag-hRIPK3
Plasmid#112495PurposeMammalian expression of 3xFlag-hRIPK3DepositorInsertRIPK3 (RIPK3 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac-G13-sensor
Plasmid#137809PurposeA FRET sensor for G13 activation, encoded on a single plasmidDepositorInsertGalpha13 sensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-UC-Cas9-T2A-mCherry
Plasmid#135014PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to C-terminus of Cas9, linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
UseTags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianMutation126aa domain from HSV-1 UL12 fused to the C-termi…PromoterCBhAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-RNF126
Plasmid#138643PurposeBacterial expression of GST-RNF126 fusion proteinDepositorInsertRNF126 (RNF126 Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-134
Plasmid#118520PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM5-Flag
Plasmid#180410Purposelentiviral transduction of human TGM5 (with a C-terminal Flag tag) geneDepositorInsertTGM5 (TGM5 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-136
Plasmid#118522PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-PLXNB2
Plasmid#86237PurposeLentivirus for expression of human Plexin-B2DepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralTagsExpressionMammalianMutationfull length PLXNB2, CRISPR resistantPromoterhuman PGK promoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC25A19
Plasmid#132263PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC25A19 (SLC25A19 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
mVenus-Peroxisomes-2
Plasmid#54692PurposeLocalization: Peroxisomes, Excitation: 515, Emission: 528DepositorTypeEmpty backboneUseTagsPeroximal Targeting Signal 1 (PTS1) and mVenusExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry
Plasmid#135013PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
UseTags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianMutation126aa domain from HSV-1 UL12 fused to the N-termi…PromoterCBhAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)
Plasmid#134333PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA17_Efa
UseTagsNLS(SV40)ExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)
Plasmid#134337PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA16_Efa
UseTagsNLS(SV40)ExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b-EC12WTC9AAvi
Plasmid#47925PurposeExpresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.DepositorInsertE-Cadherin EC12
UseTags6xHis, Avi Tag, and TEV CleavageExpressionBacterialMutationchanged Cysteine 9 to AlaninePromoterT7Available sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3E-EEA1
Plasmid#109560PurposeMultisite gateway vector for 5' tagging with early endosome associated 1DepositorInsertearly endosome associated 1 (eea1 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-CKMT2
Plasmid#23746DepositorInsertCKMT2 (CKMT2 Human)
UseGateway donor vectorTagsExpressionMutationS6PPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-PANK4
Plasmid#23419DepositorInsertPANK4 (PANK4 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen0.7
Plasmid#78176PurposeExpresses rsGreen0.7 in E. coli strains.DepositorInsertrsGreen0.7
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
SFFV-TCF3-Brd 213
Plasmid#219164PurposeTranscription factor TCF3 with a specific barcode assigned.DepositorInsertTCF3 (TCF3 Human)
UseLentiviralTagsExpressionMutationCodon optimizedPromoterThe spleen focus-forming virus (SFFV) promoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_SNAP-tag2
Plasmid#226512PurposeBacterial cell expression of SNAP-tag2DepositorInsertSNAP-tag2
UseTagsHis-tag (10x) and Strep-Tag II,ExpressionBacterialMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only