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Plasmid#208857PurposeSSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only
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pAAV-Ef1a-DO-ChETA-TdTomato-WPRE-pA
Plasmid#37756PurposeExpresses ChETA-TdTomato in cells lacking Cre (Cre-Off), for optogenetic excitationDepositorInsertChETA-TdTomato
UseAAV and Cre/Lox; Cre-offTagsTdTomatoExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
S12.1_Empty_Bridge
Plasmid#127670PurposeAmpicillin resistant multiplexing backbone without CRY2HA and mCherryDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-ChETA-EYFP-WPRE-pA
Plasmid#37086PurposeExpresses ChETA-EYFP in cells lacking Cre (Cre-Off), for optogenetic excitationDepositorInsertChETA-YFP
UseAAV and Cre/Lox; Cre-offTagsEYFPExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
GOMO-GFP-LUC
Plasmid#60976PurposeRapid selection of recombinant marker-free bioluminescent rodent malaria parasitesDepositorInsertsGFP-Luciferase fusion
mCherry
hDHFR-yFCU
UseTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-BclXL-Blasticidin
Plasmid#120718PurposeExpresses mitochondrial membrane-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertBl2-like protein 1, isoform Bcl-X(L)
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutation33 c-terminal amino acidsPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF-XMAS-2xStop
Plasmid#164412PurposeTransient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsT2A-GFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
GOMO-GFP
Plasmid#60975PurposeRapid selection of recombinant marker-free fluorescent rodent malaria parasitesDepositorInsertsGFP
mCherry
hDHFR-yFCU
UsePlasmodium gene targetingTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLBM0090
Plasmid#172824PurposeLevel 2 module containing mCHERRY-nVENUS HSP21-cVENUSDepositorInsert[35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA
Plasmid#37089PurposeExpresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitationDepositorInsertChETA-TdTomato
UseAAV and Cre/Lox; Cre-offTagsTdTomatoExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCASP-SptP120-3m160-HilA
Plasmid#153325PurposeTriggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinoseDepositorInsertSptP120-3m160 DARPin
UseSalmonella expressionTagsExpressionMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_MYOD1
Plasmid#64136PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.DepositorInsertsgRNA1_MYOD1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA2_MYOD1
Plasmid#64137PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.DepositorInsertsgRNA1_MYOD1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-NpHR3.0-eYFP-WPRE-pA
Plasmid#37088PurposeExpresses NpHR3.0-EYFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic inhibitionDepositorInsertNpHR3.0-EYFP
UseAAV and Cre/Lox; Cre-offTagsEYFPExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
sgRNA4_MYOD1
Plasmid#64139PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA1_MYOD1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
XW20
Plasmid#65836PurposePunc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
UseTagsExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFIN-EF1a-CHER-WPRE
Plasmid#44353DepositorInsertEF1a-CHER
UseLentiviralTagsExpressionMutationPromoterhuman elongation factor -1a (pos 2526-3969)Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
sgRNA3_MYOD1
Plasmid#64138PurposePhotoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA1_MYOD1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Py2087ACR1
Plasmid#153032PurposeAnion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).DepositorInsertPy2087ACR1
UseTagsmCherryExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHEE401_GV_MOD
Plasmid#194874PurposemCherry selection in Arabidopsis seeds in T1 and T2DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoteregg cell-specificAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only