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Plasmid#59355PurposeKnocks down TACC3 in mammalian cells via shRNA and co-expresses GFP.DepositorInsertEGFP
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
3NES-CS(G)-tTA-CS(G)-3NES
Plasmid#137836PurposeSoluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequencesDepositorInsert3NES-CD(G)-tTA-CS(G)-3NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSW609 - N001
Plasmid#115993Purposeintermediate vector for GreenGate cloning method, contains modules from A-FDepositorInsertH-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseUnspecifiedTagsHDEL and SP(ER)ExpressionMutationPromoterAvailable sinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-C3-beta-spectrin
Plasmid#202685Purposefluorescent protein tagged beta-spectrinDepositorInsertEGFP:SPTB(1-2364) (SPTB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-tdTomatof
Plasmid#104060PurposeAlso known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracingDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)
Plasmid#55220DepositorTypeEmpty backboneUseTagsHis6 and MBP, Asn10ExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-eNpHR 3.0-EYFP
Plasmid#26775Purpose3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by human Synapsin I promoter for optogenetic inhibitionDepositorInserteNpHR 3.0
UseLentiviralTagsEYFPExpressionMammalianMutationTrafficking Signal (TS) ER Export SignalPromoterhSynAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot7
Plasmid#37325DepositorInsertHsNot7 (CNOT7 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-mRFP
Plasmid#128126PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBen (benzoate responsive promoter)Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9
Plasmid#78903PurposeExpresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cellsDepositorInsertdCas9
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT2dR3-proB
Plasmid#122595PurposeBE PACE selection, express T7 RNA polymerase with degron-GTCCA targetDepositorInsertT7 RNA polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-Klentaq
Plasmid#157862PurposePlasmid for the aTc-inducible expression of the KlentaqDepositorInsertKlentaq
UseTags6xHisExpressionBacterialMutationPromotertet PAAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL638
Plasmid#189831PurposeThe T-DNA backbone of nCas12j2 for genome editing in riceDepositorTypeEmpty backboneUseCRISPR; T-dna cloneTagsExpressionMutationPromoterZmUbi1Available sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMJ915v2+Nterm Spe1 +Cterm Age1 site
Plasmid#88914PurposeFor expression of modified SpyCas9 in e.coli.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
HP138-neo
Plasmid#134247PurposeCas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selectionDepositorInsertContains Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL1749 (pDonor, R*MmeI)
Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP341 pHAGE2 CMV TagBFP-3xFLAG
Plasmid#199391PurposeLentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cellsDepositorInsertTagBFP-3xFLAG
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP924 pHAGE2 CMV GFP
Plasmid#199392PurposeLentiviral transfer plasmid for constitutive expression of a GFP in mammalian cellsDepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 human TBC1D7 (19-293)
Plasmid#132617Purposeexpresses human TBC1D7 (19-293) in E. coliDepositorInsertTBC1D7 (19-293)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL2621 (pCas8_Vch)
Plasmid#200893PurposeExpresses human codon-optimized V. cholerae Cas8 from a CMV promoter.DepositorInsertVchCas8
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only