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Plasmid#196599PurposeMammalian expression of Td-CGBEDepositorInsertbpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
UseTags6*HisExpressionMammalianMutationTadA-8e (N46L); S. pyogenes Cas9 (D10A)PromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2342
Plasmid#142041PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMITF (MITF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAD12
Plasmid#34832DepositorInsertNone
UseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-CytERM-mCherry-(F30-2xPepper)10
Plasmid#129403PurposeCytERM-mCherry mRNA reporter tagged with (F30-2xPepper)10DepositorInsertCytERM-mCherry-(F30-2xPepper)10
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLG028 (hhe)
Plasmid#157906PurposeExpresses the hhe defense systemDepositorInserthhe (DUF4011-helicase-Vsr-DUF3320)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GalNAc-T2-GFP-ESCargo(FTV)
Plasmid#140163PurposeExpresses a regulatable secretory cargo for mammalian cells and a Golgi markerDepositorInsertGalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Synthetic, Human)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termiā¦ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-UBE2E1 C131A in pcDNA3.1
Plasmid#118214PurposeExpresses HA-UBE2E1 C131A catalytic mutant in mammalian cellsDepositorInsertUBE2E1 (UBE2E1 Human)
UseTagsHAExpressionMammalianMutationchanged cysteine 131 to alaninePromoterAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG032 (ppl)
Plasmid#157910PurposeExpresses the ppl defense systemDepositorInsertppl (PHP-ATPase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-GCaMP6s
Plasmid#135418PurposeCan be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.DepositorInsertGCaMP6s
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
mito NAD+ biosensor
Plasmid#186791PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondriaDepositorInsertmito NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only