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SPLICS Mt-ER- Po Short P2A

Plasmid

#164118

Purpose

Detect the short Peroxisomes-mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP Mt-ER- Po Short P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-MXT GIRK2

Plasmid

#20675

Depositor

Henry Lester

Article

Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

Insert

Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)

Use

Xenopus expression

Tags

3' UTR Xenopus B- globin and 5' UTR Xen…

Expression

Mutation

A369V

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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EF1a_SPI1_P2A_Hygro_Barcode

Plasmid

#120482

Purpose

Barcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPI1 (SPI1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- Po Long P2A

Plasmid

#164117

Purpose

Detect the long Peroxisomes-mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP Mt-ER- Po Long P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

Plasmid

#107319

Purpose

Expresses SpCas9 fused to dSaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-iLIDx6

Plasmid

#103779

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

mCherry-iLIDx6

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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nLuc-PTHR1

Plasmid

#162577

Purpose

Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells

Depositor

Sam Gellman

Article

Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

Insert

Parathyroid Hormone Receptor 1 (PTH1R Human)

Use

Tags

Nanoluc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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FL tension sensor module

Plasmid

#101170

Purpose

Expresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.

Depositor

Carsten Grashoff

Article

Ringer et al Nat Methods. 2017 Nov;14(11):1090-1096.

Insert

YPet(short)-FL-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-6XPRE-GFP

Plasmid

#206171

Purpose

pMVP Gateway destination vector (3rd generation lentiviral vector) containing 6 consensus PGR binding sites upstream of a minimal 2YBTATA promoter

Depositor

Renee van Amerongen

Article

Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

Insert

6X PRE-eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

2YBTATA

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-EN1082_Rad21_STOP

Plasmid

#156450

Purpose

For transient expression of Cas9 and sgRNA targeting mouse RAD21 stop codon

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

Cas9, RAD21 (Rad21 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pJB332

Plasmid

#115949

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 TetR-Gly9-DDX6 (contains SapI)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTW122

Plasmid

#115962

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 5'-2xTetApt-mVenus-PEST

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-Akt-STOPS

Plasmid

#175009

Purpose

Plasma membrane-targeted Akt-STOPS.

Depositor

Jin Zhang

Article

Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

Insert

Lyn-mCherry-Akt-STOPS

Use

Tags

N-terminal targeting sequence from Lyn kinase and…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_mRuby2

Plasmid

#113894

Purpose

Mammalian (inducible) expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

monomeric Ruby2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 K720A

Plasmid

#19939

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 K720A (CUL1 Human)

Use

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a NEDD8 modification mutant

Promoter

Available since

April 17, 2009

Availability

Academic Institutions and Nonprofits only
HOXA7 (murine) His-tag pET

Plasmid

#8491

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA7 (Hoxa7 Mouse)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG; makes protein by …

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCEP-WAF1-AS

Plasmid

#16448

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 antisense (CDKN1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pl-Synapsin-YFP-NaVII-III

Plasmid

#91246

Purpose

Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)

Depositor

Matthew Grubb

Article

Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.

Insert

Nav1.2 (Scn2a Rat)

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Mutation

The insert codes only for the intracellular loop …

Promoter

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 Y42A/M43A

Plasmid

#19940

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 Y42A/M43A (CUL1 Human)

Use

Tags

HA2

Expression

Mammalian

Mutation

CUL1 with a SKP1 binding mutant

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pGLACTE-PseudoHT51

Plasmid

#99257

Purpose

Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) within the 5'UTR of FMR1. Internal control with synthetic primer sequence for capillary electrophoresis

Depositor

Karen Usdin

Article

Hayward et al J Mol Diagn. 2017 Nov;19(6):828-835. doi

Insert

FMR1 (FMR1 Synthetic, Human)

Use

Tags

Expression

Mutation

Contains synthetic, non-human, primer sequence do…

Promoter

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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