Addgene

Showing: 5541 - 5560 of 11785 results

Results per page:

Sort results by:

pUC57mini ZM249M P90A _env eGFP

Plasmid

#132939

Purpose

pUC57mini ZM249M _env eGFP containing P90A mutant capsid sequence

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

HIV-1ZM249M provirus (P90A) with env deletion, GFP

Use

Lentiviral

Tags

Expression

Mutation

P90A

Promoter

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2- Empty control -SV40-Luc

Plasmid

#26280

Purpose

Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
penaav-tnt-3xha-p2a-gfp

Plasmid

#175090

Purpose

For making AAV9

Depositor

William Pu

Article

Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
3xFLAG-SENP6

Plasmid

#42887

Depositor

Edward Yeh

Article

Dou et al Mol Cell. 2010 Aug 13. 39(3):333-45.

Insert

SENP6 (SENP6 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Please see depositor comments

Promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLX-G4E1b-R1DESTR2-MIFE-PGK-mCherry

Plasmid

#114322

Purpose

Mifepristone-inducible lentiviral backbone for Gateway cloning of Gene insert together with constitutive mCherry expression

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Brachyury-eGFP

Plasmid

#21063

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Brachyury promoter

Use

Lentiviral

Tags

eGFP

Expression

Mutation

Promoter

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170809

Purpose

Lentiviral vector to co-express an spsgRNA with NLS-mNeonGreen

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV125

Plasmid

#63191

Purpose

pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFPI7

Plasmid

#83555

Purpose

Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

CUP1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 alpha 1A AR

Plasmid

#45760

Depositor

Robert Lefkowitz

Article

Schwinn et al J Biol Chem. 1990 May 15;265(14):8183-9.

Insert

alpha 1A AR (Adra1a Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pMXs_NMNAT3-FLAG

Plasmid

#133261

Purpose

Express FLAG-tagged NMNAT3 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

NMNAT3 (NMNAT3 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetOminiCMV-Sox2

Plasmid

#136612

Purpose

Dox-inducible lentiviral vector expressing mouse Sox2

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Pet15b-Endosomal Escape Control

Plasmid

#186552

Purpose

Negative Control confirming the need for a CPP peptide in mediating internalization

Depositor

Jean Gariepy

Article

Mohammed et al J Control Release. 2012 Nov 28;164(1):58

Insert

6H-ETA-GFP

Use

Tags

6xHis and EGFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pActin-miRFP670nano3

Plasmid

#184665

Purpose

Human beta-actin labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-human beta-actin

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAT024

Plasmid

#171636

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-BFP
mTagBFP2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG and U6

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
3XFlag DVL2 (∂DIX)

Plasmid

#24805

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of the N-terminal DIX domain

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
OmpA-mNG-mCh

Plasmid

#124216

Purpose

Periplasmic OM bound mNeongreen mCherry tandem, ~16 % energy transfer. pNM004 - pTHV037-OmpA-SA1-177-(SA-1)-LEDPPAEL-mNG-mCh

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al MBio. 2017 Sep 12;8(5). pii: mBio.01089-

Insert

OmpA-mNG-mCh

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
3XFlag DVL2 (∂361-736)

Plasmid

#24806

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

DVL2 (DVL2 Human)

Use

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of aa 361-736

Promoter

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pU6-Decoy

Plasmid

#80324

Purpose

Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

Decoy sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pConRef-2H12

Plasmid

#221182

Purpose

cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen
mScarlet-I

Use

Tags

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

Constitutive and None (same as cdGreen)

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge