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Plasmid#166840PurposeExpresses ER localized Vac8-GFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorUseTagsGFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only
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pGEX-2T-RNAPII-CTD wt #14-HA
Plasmid#160688PurposeExpression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.DepositorInsertRNA Polymerase II CTD
UseTagsExpressionBacterialMutationC terminal domain onlyPromoterAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(L313D I314K)
Plasmid#121390PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationLeucine 313 to Aspartic acid; Isoleucine 314 to L…PromoterADH1 promoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES260-sc.eEF2 K509A
Plasmid#85120Purposeyeast expression of mutant EEF2DepositorUseTags6xHisExpressionYeastMutationK509APromoterGAL1Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRevLCav2FeS
Plasmid#60808Purposeto disrupt iron sulfur cluster in yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
UseTagsExpressionYeastMutationcontains 647 C-terminal amino acids and 335 bp of…PromoterAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-Nop4 L306P
Plasmid#169262PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 L306PDepositorInsertNop4 L306P (NOP4 Budding Yeast)
UseTags3xFLAGExpressionYeastMutationp.(Leu306Pro)PromoterGPDAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-RBM28 WT
Plasmid#169265PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 WTDepositorInsertRBM28 WT (RBM28 Human)
UseTags3xFLAGExpressionYeastMutationPromoterGPDAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-RBM28 L351P
Plasmid#169266PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 L351PDepositorInsertRBM28 L351P (RBM28 Human)
UseTags3xFLAGExpressionYeastMutationp.(Leu351Pro)PromoterGPDAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pHIPH4
Plasmid#117685PurposeHansenula polymorpha expression plasmidDepositorInsertAlcohol Oxidase promoter
UseTagsExpressionYeastMutationPromoterAlcohol OxidaseAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILAC3
Plasmid#185890PurposeIn vivo gene amplification (HamAmp) of lycopene synthetic module at RPL25 locusDepositorInsertsee comments
UseTagsExpressionYeastMutationERG20 F96C; Xd.CRtYB E83KPromoterAvailable sinceSept. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIT6EG7ml
Plasmid#185886PurposeIn vivo gene amplification (HamAmp) of limonene synthetic module at RPL25 locus (14 copy).DepositorInsertsee comments
UseTagsExpressionYeastMutationERG20 F96W N127WPromoterAvailable sinceSept. 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
p42Hyg 1-F GW
Plasmid#58551PurposeMulticopy vector with Hygromycin resistance marker for single Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDB24
Plasmid#91959Purposeempty expression vector for 10His-SUMO tag protein purificationDepositorTypeEmpty backboneUseTags10-His and Smt3ExpressionBacterial and YeastMutationPromoterT7Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
p41Hyg 1-F GW
Plasmid#58547PurposeCEN vector with Hygromycin resistance marker for single Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS426 TEF mCherry
Plasmid#129433Purposeexpress mcherryDepositorInsertmcherry
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJA149
Plasmid#128524PurposeExpresses mutant Ber e 1 in PichiaDepositorInsertmutant Ber e 1
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
p42Hyg 3-F GW
Plasmid#58559PurposeMulticopy vector with Hygromycin resistance marker for triple Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS415-KEX2
Plasmid#10996DepositorInsertKEX2 (KEX2 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS415-SPT6
Plasmid#10993DepositorInsertSPT6 (SPT6 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
RPS1B-GFP (95)
Plasmid#24039DepositorInsertRPS1B (RPS1B Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only