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MDM4_CRISPRi_gRNA_2

Plasmid

#195133

Purpose

gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 CRISPRi gRNA 2 (MDM4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_CRISPRi_gRNA_1

Plasmid

#195132

Purpose

gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 CRISPRi gRNA 1 (MDM4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_Deletion_Downstream_gRNA_1

Plasmid

#195136

Purpose

gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Downstream gRNA 1 (MDM4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pITGA5_1745G>U

Plasmid

#173812

Purpose

mir218 binding site2 point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

NT1745 ITGA5 point mutation (ITGA5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

AmpR

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pITGA5_Q853L

Plasmid

#174008

Purpose

Fibronectin receptor binding site point mutation

Depositor

Andrew M. Siedlecki

Article

Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

Insert

FibronR ITGA5 point mutation (ITGA5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

AmpR

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-CMVtight-PLXNB2 iOE

Plasmid

#176849

Purpose

lentiviral vector for doxycycline-inducible PLXNB2 overexpression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Tags

Expression

Mutation

full length PLXNB2

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-hPLXNB2_PGK_Neo

Plasmid

#176848

Purpose

lentiviral vector for constitutive PLXNB2 overexpression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Tags

Expression

Mutation

full length PLXNB2

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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EIF2B3-C424G

Plasmid

#172091

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.1270T>G

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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EIF2B3-V398fs

Plasmid

#172090

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.1193-1194delTG

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-S369fs

Plasmid

#172089

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.1106_1113del

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-I346T

Plasmid

#172088

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.1037T>C

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-H341Q

Plasmid

#172087

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.1023T>G

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-A322G

Plasmid

#172086

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.965C>G

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-R312Q

Plasmid

#172085

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.935G>A

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-Q236E

Plasmid

#172084

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.706C>G

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-R225Q

Plasmid

#172083

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.674G>A

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-L168P

Plasmid

#172082

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.503T>C

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-Q136P

Plasmid

#172081

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.407A>C

Promoter

CMV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-R91H

Plasmid

#172080

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.272G>A

Promoter

CMV

Available since

July 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-A87V

Plasmid

#172079

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Use

Tags

Expression

Mammalian

Mutation

c.260C>T

Promoter

CMV

Available since

July 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge