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8xNFAT-ZsG-hCD8

Plasmid

#153417

Purpose

NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12

Plasmid

#101285

Purpose

Lentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFP

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-H-Ras

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

H-Ras V12

Promoter

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-TetON-GFP

Plasmid

#115495

Purpose

Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

Depositor

Catherine Verfaillie

Article

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-RsACE2

Plasmid

#159665

Purpose

Expresses Rhinolophus sinicus ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pE-cadherin-GFP-N-WASP deltaVCA

Plasmid

#101283

Purpose

Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

delta VCA domain

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-MIR302-7guides-PGK-Puro

Plasmid

#201960

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette

Depositor

Timo Otonkoski

Article

Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

Insert

MIR302-7g-PGK-Puro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF2-ABCG2-IRES-Neo

Plasmid

#25983

Depositor

Ren-he Xu

Article

Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

Insert

ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)

Use

Lentiviral

Tags

EF1-apha promoter and IRES-Neo

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-beta-HA-BRCA2

Plasmid

#133553

Purpose

Mammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORF

Depositor

David Livingston

Article

Chen et al Mol Cell. 1998 Sep;2(3):317-28.

Insert

BRCA2 (BRCA2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57.1 _3xHA-Ago2

Plasmid

#73538

Purpose

Inducible lentiviral expression of Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet ON

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pTFG001-ACE2(WT)-HA

Plasmid

#191569

Purpose

ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

Angiotensin Converting Enzyme II (ACE2 Human)

Use

Lentiviral and RNAi

Tags

HA tag

Expression

Mammalian

Mutation

Codon optimized for expression in human cells

Promoter

CMV

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo-His6

Plasmid

#212819

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Use

Tags

CUbo-6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo

Plasmid

#212821

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Use

Tags

CUbo

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K63R)

Plasmid

#212822

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R) in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Use

Tags

CUbo(K63R)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 _3xHA-Ago2_RNAmut

Plasmid

#73539

Purpose

Inducible lentiviral expression of Ago2_RNAmut

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Small RNA binding mutant PAZ domain Y311A and F31…

Promoter

TRE promoter, Tet ON

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1 _3xHA-Ago2_CATmut

Plasmid

#73540

Purpose

Inducible lentiviral expression of Ago2_CATmut

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

Catalytic mutant PIWI domain D670A

Promoter

TRE promoter, Tet ON

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-His6

Plasmid

#212820

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-His6 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Use

Tags

CUbo(K63R)-6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo-GFP

Plasmid

#212815

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Use

Tags

8xHis and CUbo-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K48R)-GFP

Plasmid

#212816

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R)-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Use

Tags

CUbo(K48R)-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-GFP

Plasmid

#212817

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Use

Tags

CUbo(K63R)-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K48R K63R)-GFP

Plasmid

#212818

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R K63R)-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Use

Tags

CUbo(K48R/K63R)-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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