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Plasmid#113893PurposeMammalian (inducible) expression of cytosolic mVenusDepositorInsertmonomeric Venus
UseLentiviralTagsExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAV1-K*R-HA
Plasmid#27765DepositorInsertcaveolin-1, lysine null mutant (CAV1 Human)
UseTagsHAExpressionMammalianMutationAll lysines mutated to arginines (K*R)PromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2 EGFP sgRNA2
Plasmid#164933PurposeExpresses EGFP sgRNA2 in mammalian cellsDepositorInsertsgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
UseLentiviralTagsExpressionMutationPromoterEFS promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-2XPRE-GFP
Plasmid#206169PurposepMVP Gateway destination vector (3rd generation lentiviral vector) containing 2 consensus PGR binding sites upstream of a minimal 2YBTATA promoterDepositorInsert2X PRE-eGFP
UseLentiviralTagsExpressionMutationPromoter2YBTATAAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)
Plasmid#71719PurposeExpressing a GFP-tagged degron in the soma of C. elegansDepositorInsertauxin-responsive protein IAA17 (AXR3 Mustard Weed)
UseTagsEmGFPExpressionWormMutationminimal degron sequence (71-114aa) with start cod…Promotereef-1A.1 (eft-3)Available sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
LDB-14-p65
Plasmid#158985PurposeExpresses LDB-14 fused with a C-terminal p65 transcriptional activation domain in mammalian cellsDepositorInsertLDB-14-p65
UseTagsV5 tag , 6xHis tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
HsCLYBL(D320A)-FLAG
Plasmid#111291Purposemammalian expressionDepositorInserthuman CLYBL D320A (CLYBL Human)
UseTagslinker and FLAG tag: TGGGSGGSGGSMDYKDDDDKExpressionMammalianMutationD320APromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Actin-7
Plasmid#57339PurposeLocalization: Actin, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertActin (ACTB Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-AC3CtermGenomic-mCherry
Plasmid#105250Purposemouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3DepositorInsertmCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SARS-CoV-2 NSP13
Plasmid#176492PurposeExpresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.DepositorInsertSARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
UseTagsHis6 and T7ExpressionBacterialMutationI570 from SARS-CoV NSP13 was mutated to V to conv…PromoterT7Available sinceJan. 10, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEMHE-mEGFP-Map4
Plasmid#105571PurposeT7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNADepositorInsertMAP4 (Map4 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationPromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l4-G'
Plasmid#209124PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l4-G'DepositorInsertSUMO-A'-l4-G'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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Vimentin - APEX2 in pECFP
Plasmid#66170PurposeAPEX2 fused to the C-terminus human vimentinDepositorInserthuman Vimentin - APEX2 (VIM Synthetic, Human)
UseTagsFlag epitope tag (DYKDDDDK)ExpressionMammalianMutationAPEX2 contains 4 mutations relative to wt soybean…PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmiHiFiCas9
Plasmid#164832PurposemiHiFiCas9 expressing plasmidDepositorInsertmiHiFiCas9 (contains Brex27 at sequence 5031-5138).
UseCRISPRTagsFLAG and NLSExpressionMammalianMutationchange R 691 to APromoterCMVAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB_Cdc42-TomKat-sensor-CAAX
Plasmid#172473PurposePiggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.DepositorInserttdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
UsePiggybac transposonTagspolybasic and CAAX motif from Kras, tdKatushka2, …ExpressionMammalianMutationPromoterCAGAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK198 (GAL-OsTIR1)
Plasmid#140655PurposeGAL-OsTIR1(WT)DepositorInsertOsTIR1(WT)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSP2292
Plasmid#70707PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-metap2
Plasmid#100510PurposeExpresses FLAG tagged metap2 in mammalian cells to make lentivirusDepositorInsertMETAP2 (METAP2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Flag-hOct4
Plasmid#20072DepositorInserthOct4 (POU5F1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only