Addgene

Showing: 5521 - 5540 of 22746 results

Results per page:

Sort results by:

GFP-LacI-polylinker

Plasmid

#179271

Purpose

Cloning of GFP-LacI fusion proteins for LacI/LacO tethering

Depositor

Lienhard Schmitz

Article

Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

Type

Empty backbone

Use

Tags

GFP and LacI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
C122-E11: eGFP

Plasmid

#162893

Purpose

Standard Gateway entry clone encoding eGFP fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

enhanced Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCXLE-dCas9GFP-shP53

Plasmid

#102906

Purpose

EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

dCas9GFP-shP53

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-PTEN C124A

Plasmid

#50520

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

C124A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pLJM1-EGFP-BarcodeV2

Plasmid

#78632

Purpose

GESTALT V2 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT V2 insert

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pIVT-EGFP-mouseCENP-C

Plasmid

#174444

Purpose

in vitro transcription template for mouse CENP-C (N-ter EGFP tagged)

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Centromere protein C

Use

In vitro transcription template

Tags

EGFP

Expression

Mutation

Promoter

T7 SP6

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
tdp43-EGFP construct11

Plasmid

#28204

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa193-274 only

Promoter

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
GFP-Braf R188L

Plasmid

#118330

Purpose

Expression of human Braf

Depositor

Philip Stork

Article

Li et al J Biol Chem. 2013 Sep 20;288(38):27646-5

Insert

Braf R188L (BRAF Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

R188L

Promoter

CMV

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG423GAL-EGFP-ccdB

Plasmid

#14317

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
TAP-GFP-Amotl2

Plasmid

#112835

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Insert

Amotl2 (Amotl2 Mouse)

Use

Tags

GFP and TAP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CD8a-hnRNP-M-EGFP

Plasmid

#86054

Purpose

A mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

CD8a luminal and transmembrane domians along with…

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1 VAMP2 R125TAG

Plasmid

#69876

Purpose

express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP

Depositor

Silvio Rizzoli

Article

Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do

Insert

VAMP2-GFP (Vamp2 Rat)

Use

Synthetic Biology

Tags

GFP

Expression

Mammalian

Mutation

Amber stop codon in the linker between VAMP2 and …

Promoter

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
pRVdG-1mOrange2-5PSD95EGFP

Plasmid

#52482

Purpose

Expresses mOrange2 and PSD-95-EGFP

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mOrange2
PSD-95-EGFP

Use

Rabies virus

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pKEN GFP mut3

Plasmid

#20410

Depositor

Raphael Valdivia, Stanley Falkow

Article

Cormack et al Gene. 1996 . 173(1 Spec No):33-8.

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

S65G S72A

Promoter

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only
pAG306GPD-EGFP-ccdB

Plasmid

#14308

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Epac1 SAAX

Plasmid

#118313

Purpose

Expression of human Epac1

Depositor

Philip Stork

Article

Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

Insert

Epac1 SAAX (RAPGEF3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 13 - CMVp_U6p_27nt1_GFP(+3)_RFP(+2)

Plasmid

#81252

Purpose

Mutation based toggling reporter (MTBR)

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Mutation Based Toggling Reporter (MBTR)

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pORANGE CACNG2-GFP KI

Plasmid

#131504

Purpose

Endogenous tagging of Tarpγ2/Stargazin: Intramolecular (amino acid position: S265)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng2, Cacng2 Mouse, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-mApg5(K130R)

Plasmid

#22959

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

130 Lysine to Arginine

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
DsRed2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176988

Purpose

Mammalian expression vector encoding DsRed2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

DsRed2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge