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Plasmid#171359PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFP.R371
Plasmid#138241PurposeExpresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
[Bla][IRES][GFP]
Plasmid#97193PurposeVector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.DepositorInsertsbeta-Lactamase
GFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-Cas12j2
Plasmid#173924PurposeGateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLSDepositorInsertNLS-Cas12j2-NLS
UseCRISPRTagsNLSExpressionPlantMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Casp2
Plasmid#50023PurposepENTR vector for mouse Caspase-2DepositorInsertCasp2 (Casp2 Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFP.R400
Plasmid#138175PurposeExpresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW178 - pXPP
Plasmid#115987Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpXPP
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMC0x_gRNA_Pos4_Ptet
Plasmid#179335PurposegRNA Position Vector 4, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 camDepositorInsertPtet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pV1539
Plasmid#111434PurposeGenedrive entry vector for Candida albicansDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 MOV10 K530A
Plasmid#51713Purposeplasmid contains the coding sequence of MOV10 K530ADepositorInsertMOV10 K530A (MOV10 Human)
UseEntry vectorTagsExpressionMutationK530APromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E-mCherry-NS-MCS
Plasmid#75179PurposemCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions.DepositorInsertmCherry-NS-MCS
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A_no_ccDB_mUty
Plasmid#158570PurposeV5-tagged sequence of mUty cloned into entry vector for recombinationDepositorInsertMus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBW103ParsR-Amp30C
Plasmid#78638PurposepBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-tDepositorInsert30hrpR-30hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC0x_gRNA_Pos5b2_Ptet
Plasmid#179342PurposegRNA Position Vector 5b2, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 camDepositorInsertPtet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-OSF-TRIM5-21R
Plasmid#79063PurposeGateway entry vector for cloning and propagation in DH5alphaDepositorInsertOSF-TRIM5-21R
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterT7Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-nlsGal80(opt)
Plasmid#51618Purposevertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stopDepositorInsertGal80(opt) (GAL80 Budding Yeast)
UseVertebrate expressionTagsNLSExpressionMutationcodon optimizedPromoterAvailable sinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Dest_R1-R3
Plasmid#140878Purpose166571: Multisite gateway destination vector for in vitro transcription of RNA using T3. Accepts one pME and one p3E entry clone.DepositorInsertccdb/CAM
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-002
Plasmid#78689PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-mKOFP2-CAAX
Plasmid#75155PurposeMembrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.DepositorInsertmKOFP2-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only