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Plasmid#210485PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only
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pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)
Plasmid#210487PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_EmGFP
Plasmid#113892PurposeMammalian (inducible) expression of cytosolic EmGFPDepositorInsertEmerald GFP
UseLentiviralTagsExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-4031NES
Plasmid#105241PurposeA Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.DepositorInsertAMPK-EV
UseTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHLSec2-FN-YPet
Plasmid#65421PurposeExpresses in mammalian cells human plasma fibronectin with YPet inserted between FNIII domains 6 and 7DepositorInserthuman plasma fibronectin with YPet inserted between FNIII domains 6 and 7 (FN1 Human)
UseTagsYPetExpressionMammalianMutationPromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYFAC-CH3
Plasmid#168979PurposeAMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldADepositorTypeEmpty backboneUseSynthetic Biology; Heterologous expression in asp…TagsExpressionMutationPromoterAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgRNA(lib)-puro
Plasmid#119976PurposeLenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE
Plasmid#121966PurposeExpresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.DepositorInsertscFv-GCN4
UseLentiviralTagssuperfold GFP-GB1-NLS-iLIDExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCas9(D10A)
Plasmid#74495PurposeNicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10ADepositorInsertCas9 D10A
UseSynthetic BiologyTagsExpressionBacterialMutationD10APromoterpTetoAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEa
Plasmid#193782PurposeExpress TadCBEa (with SpCas9) in mammalian cellsDepositorInsertTadA-CDa-SpCas9D10A
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-lck-smV5-4x6T-WPRE
Plasmid#196416PurposeAstrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertLck-smV5
UseAAV; Astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAG and GfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
cyto-CFP-FRBx5
Plasmid#103776Purposeexpresses iPOLYMER component in mammalian cellsDepositorInsertcyto-CFP-FRBx5
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pHR-PGK-ABI-dCas9-P2A-mcherry
Plasmid#121513Purposeexpression of AB1-fused-dcas9 plasmidsDepositorInsertdCas9
UseLentiviralTagsAB1 and P2A-mcherryExpressionMammalianMutationPromoterPGKAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rabbit GFP Cav 2.2 pMT2
Plasmid#58737Purposeexpression of GFP tagged rabbit Cav2.2 in mammalian cellsDepositorInsertCav2.2 (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationPromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYOD1_P2A_Hygro_Barcode
Plasmid#120464PurposeBarcoded lentiviral vector to express MYOD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYOD1 (MYOD1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cav2.1 HA pCAGGS
Plasmid#206076Purposeexpression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain IIDepositorInsertcacna1a (Cacna1a Rat)
UseTagsExpressionMammalianMutationPromoterCMV/B-actinAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-FNIP1
Plasmid#72293PurposeoverexpressionDepositorInsertFNIP1 (FNIP1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
EF1a_RUNX1_P2A_Hygro_Barcode
Plasmid#120475PurposeBarcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertRUNX1 (RUNX1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only