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pEMS1722

Plasmid

#29298

Purpose

Expression of the reporter gene was not detected in the brain and eye using LacZ.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple250 (VIP Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Expression

Mutation

Promoter

Available since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR223-PRDX5K85R

Plasmid

#98694

Purpose

Gateway cloning of human PRDX5-K85R

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

K85R

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5K83R

Plasmid

#98693

Purpose

Gateway cloning of human PRDX5-K83R

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

K83R

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR223-PRDX5E80K

Plasmid

#98692

Purpose

Gateway cloning of human PRDX5-E80K

Depositor

Mohan Babu

Article

Malty et al Cell Syst. 2017 Nov 7. pii: S2405-4712(1

Insert

PRDX5 (PRDX5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E80K

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV4 p50

Plasmid

#21965

Depositor

Warner Greene

Article

Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

Insert

NF-kB p50 subunit (NFKB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-Intersectin Short

Plasmid

#47394

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

Intersectin short (ITSN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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AID-NG-CBE

Plasmid

#227416

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AID-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-FLEX-CybSEP2-WPRE

Plasmid

#207661

Purpose

Monitoring neuropeptide release

Depositor

Sung Han

Article

Kim et al bioRxiv 2023.01.19.524797

Insert

Cytochrome b-561 (Cyb561 Mouse)

Use

AAV

Tags

Expression

Mutation

Histidine 86 and 159 to alanines, inserted 2 X SE…

Promoter

hSynapsin

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCH1/RAD51o

Plasmid

#102562

Purpose

Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

Depositor

Maria Spies

Article

Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

Inserts

Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
E. coli groE operon

Use

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli Expression

Promoter

T7

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-fDIO-GFP-IRES-tTA

Plasmid

#118026

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-fDIO-GFP-IRES-tTA

Use

AAV

Tags

Expression

Mutation

See depositor comments below

Promoter

TRE

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA5-PSMA6-PSMA7

Plasmid

#217894

Purpose

Insect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA5 (PSMA5 Human)
PSMA6 (PSMA6 Human)
PSMA7 (PSMA7 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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Flag Intersectin Short (human)

Plasmid

#47392

Depositor

Peter McPherson

Article

Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

Insert

Intersectin short (ITSN1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-U6-pegRNA_KCNA1-EGFP

Plasmid

#159787

Purpose

S. pyogenes pegRNA for C to A mutation at the KCNA1 site of human cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA targeting KCNA1 gene (KCNA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA_KCNA1-mcherry

Plasmid

#159788

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human KCNA1 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting KCNA1 gene (KCNA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSin-5HT-1.0

Plasmid

#172882

Purpose

make sindbis virus of 5-HT sensor GRAB_5-HT1.0 and express in cells

Depositor

J. Julius Zhu

Article

Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

Insert

5HT SENSOR

Use

Tags

Expression

Mammalian

Mutation

Promoter

sp6

Available since

Oct. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pJS699

Plasmid

#168779

Purpose

Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Insert

Spike RBD (S betacoronavirus)

Use

Tags

Cmyc

Expression

Mutation

N343Q

Promoter

GAL1

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pUDP012

Plasmid

#101167

Purpose

E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

Depositor

Jean-Marc Daran

Article

de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

Insert

HH-gRNA-HDV targetting SeILV6 in S. pastorianus

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

ScTDH3

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAS18

Plasmid

#158787

Purpose

Ribosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Ptag-168::GCaMP6m::rpl-1a

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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DJ-1 TEV site pET15b

Plasmid

#60687

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

TEV site was inserted (see comments below).

Promoter

T7

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-U6-pegRNA_FANCF-EGFP

Plasmid

#159785

Purpose

S. pyogenes pegRNA for C to A mutation at the FANCF site of human cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA targeting FANCF gene (FANCF Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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