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Showing: 5501 - 5520 of 148587 results

  1. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Use
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

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  10. pAAV-GFAP-hM3D(Gq)-mCherry

    Plasmid
    #50478
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter
    Depositor
    Bryan Roth
    Has Service
    AAV5
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50478-plasmid-map-sequence-id-166302

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  11. pCRISPRi_Mxi1_yl

    Plasmid
    #91248
    Purpose
    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    91248-plasmid-map-sequence-id-192174

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  16. pSNAP-tag (T7) Vector

    Plasmid
    #101137
    Purpose
    Expression of protein of interest with SNAP-Tag in bacterial cells
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Use
    Tags
    SNAP-tag (SNAP26b)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101137-plasmid-map-sequence-id-194162

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  20. pMN-333

    Plasmid
    #195467
    Purpose
    Lentiviral Integratable Inducible synZiFTR ZF3 + Reporter
    Depositor
    Ahmad Khalil
    Article
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Insert
    inverted[8X ZF3 BS - ybTATA - mCherry] - pSFFV - ZF3 - p65 - ERT2
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    195467-plasmid-map-sequence-id-384352

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Showing: 5501 - 5520 of 148587 results