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Plasmid#168050PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.DepositorInsertBlue light-inducible AraC dimers in Escherichia coli
UseTagsExpressionBacterialMutationPromoterJ23101**Available sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK107
Plasmid#110575PurposeBTK Type 2 promoter for golden gate assemblyDepositorInsertCP25 Promoter with RBS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti X2 Neo DEST (w18-1)
Plasmid#17390Purpose3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, NeoDepositorTypeEmpty backboneUseLentiviral and RNAi; Destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pmRE-Tn5-147
Plasmid#118526PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-LNGFR-FF3
Plasmid#11666Purpose3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferaseDepositorInsertFF3
UseLentiviralTagsLNGFRExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-131
Plasmid#118549PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTK2_005
Plasmid#123700PurposeEncodes pCAG as a Type 2 part to be used in the MTK systemDepositorInsertpCAG
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTU387
Plasmid#178981PurposepACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR arrayDepositorInsertCRISPR array from Candidatus Scalindua brodae
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-146
Plasmid#118530PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC002 - LshC2C2 locus into pACYC184
Plasmid#79151PurposeEndogenous LshC2c2 locus cloned into pACYC184DepositorInsertLshC2c2 locus
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-NEUR
Plasmid#53271PurposeContains the Erwinia herbicola (Pantoea agglomerans) Eho10 genes crtE and crtB, and the Rhodobacter capsulatus crtI gene and thereby produces neurosporene in Escherichia coliDepositorInsertcrtI
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-156
Plasmid#118570PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::MBP-NL3F10H
Plasmid#141291PurposeMBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertMBP-NLUC3F10H
UseLuciferaseTagsFlag (x3), His (x10), and MBPExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
N-BLa 1.2
Plasmid#88999PurposeBLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.DepositorInsertN-BLa 1.2 fusion protein
UseTagsFlagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-153
Plasmid#118567PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046810
Plasmid#149251PurposeBacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPbuCas13b-gRNA-NT
Plasmid#184568PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for PbuCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array witha nontargeting spacer for PbuCas13b in bacteria.
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVenusNfN
Plasmid#128564PurposeProtein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.DepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-137
Plasmid#118555PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK3a_007
Plasmid#123760PurposeEncodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK systemDepositorInsertPTS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only