We narrowed to 57,257 results for: %s

1. pCR-Flag-IKKbeta

   Plasmid

   #15465

   Depositor

   Hiroyasu Nakano

   Article

   Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

   Insert

   IkB kinase beta (Ikbkb Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

2. M_ST1n_VPR

   Plasmid

   #63799

   Purpose

   ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

   Insert

   ST1-dCas9-VPR
   

   Use

   CRISPR and Synthetic Biology

   Tags

   VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5-TEX264-deltaLRR-cSSH

   Plasmid

   #220210

   Purpose

   Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   Strep-strep-HA

   Expression

   Mammalian

   Mutation

   c.4-75del / p.2-24del

   Promoter

   CMV/TetO

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SFRS16

   Plasmid

   #156009

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SFRS16 (CLASRP Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2K7bsdUBI-mCherry-STIM1

   Plasmid

   #114178

   Purpose

   Lentiviral vector for expression of mCherry-STIM1

   Depositor

   Nicolas Demaurex

   Article

   Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry (inserted after signal peptide)

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETDuet-TFIIF

   Plasmid

   #171082

   Purpose

   Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   RAP30 (GTF2F2 Human)
   RAP74 (GTF2F1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. eGFP-proα1(I)

   Plasmid

   #119843

   Purpose

   Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α1 chain (Col1a1 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Col1a1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-TEX264-deltaLRR-His6

   Plasmid

   #220215

   Purpose

   Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   c.4-75del / p.2-24del

   Promoter

   T7

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIPZ-TIR1-HA

   Plasmid

   #172610

   Purpose

   Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   TIR1
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Tet/ON Minimal CMV promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

    Plasmid

    #172604

    Purpose

    Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Type

    Empty backbone

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG106 Lyn11-FAST

    Plasmid

    #130721

    Purpose

    Expresses FAST (also called YFAST) fused to Lyn11 sequence in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    lyn11-FAST
    

    Use

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. P-Ef1a-EGFP-T2A-RPL22-1xFlag

    Plasmid

    #170323

    Purpose

    Expresses FLAG and EGFP tagged RPL22

    Depositor

    Hemali Phatnani

    Article

    Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

    Insert

    RPL22 (RPL22 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-eGFP-NLS-RNF168

    Plasmid

    #133977

    Purpose

    mammalian expression vector of eGFP tagged wild type RNF168

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

    Promoter

    CMV

    Available since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. AAV.CBA.YFP.miR-E_shControl

    Plasmid

    #180386

    Purpose

    Producing AAV that encodes negative control shRNA with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

    Insert

    shControl
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b-His-3C-irisin

    Plasmid

    #122612

    Purpose

    Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

    Depositor

    Harold Erickson

    Article

    Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

    Insert

    irisin (ectodomain of FNDC5) (Fndc5 Mouse, Human, Rat, Bovine)
    

    Use

    Tags

    Nt: histag-3C protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 polymerase

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR-HA-IKKbeta

    Plasmid

    #15470

    Depositor

    Hiroyasu Nakano

    Article

    Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

    Insert

    IkB kinase beta (Ikbkb Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

17. Lenti-40XUAS-Luc

    Plasmid

    #166455

    Purpose

    Luciferase reporter

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    40XUAS , Luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. sh-SRF

    Plasmid

    #100797

    Purpose

    expression of shRNA targeting SRF

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

    Insert

    rat SRF
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. CLP1

    Plasmid

    #155462

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CLP1 (CLP1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-NES-NIR-GECO1

    Plasmid

    #113683

    Purpose

    Express NIR-GECO1 preferentially in neurons

    Depositor

    Robert Campbell

    Article

    Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

    Insert

    NES-NIR-GECO1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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