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Plasmid#162800PurposeYeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6DepositorInsert2_6_RNA-device
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2āTsa2ĪCPĪCR
Plasmid#83239PurposeThe catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ĪCPĪCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCpdCr
UseTagsroGFP2 fusion with Tsa2dCpdCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-hApg5-HA
Plasmid#22948DepositorInsertHomo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)
UseTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
mt deltaN20 TFB2MdeltaC3
Plasmid#205062PurposeTFB2M protein expressionDepositorInsertTFB2M (TFB2M Human)
UseTagsExpressionBacterialMutationLacks N-terminal 20 aa and C-terminal 3 aaPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-FLJ90652
Plasmid#15359DepositorInsertFLJ90652 (INO80E Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L
Plasmid#89576PurposecaCas9 integrating vector into the Neut5L locusDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD102
Plasmid#131403PurposeE. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD102
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS405 SEC66-VAC8-RFP
Plasmid#166843PurposeExpresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.DepositorUseTagsRFP and SEC66 transmembrane domainExpressionYeastMutationSEC66 transmembrane domainPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
p403 ā TDH3-MTS-mCherry-GFP1-10-His
Plasmid#177730PurposemCherry and the 1-10th Ī²-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS markerDepositorInsertmCherry-GFP1-10
UseTagsMitochondrial Targeting SequenceExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD101
Plasmid#131402PurposeE. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD101
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
ABF2-2EGFP-ter-URA3
Plasmid#184768PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertABF2 (ABF2 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYES260-sc.eEF2
Plasmid#85119Purposeyeast expression of EEF2DepositorUseTags6xHisExpressionYeastMutationPromoterGAL1Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
p414GPD-3xFLAG-Nop4 WT
Plasmid#169261PurposeS. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WTDepositorInsertNop4 WT (NOP4 Budding Yeast)
UseTags3xFLAGExpressionYeastMutationPromoterGPDAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJKW1813
Plasmid#174003PurposeExpresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeastDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
3269 pBS Bcl-2
Plasmid#8764DepositorInsertBcl-2 (Bcl2 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
BAS292 C terminal SF-GFP kanR
Plasmid#74087PurposeS.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistanceDepositorInsertSF-GFP recoded for S.pombe
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFBP-4_1.sensor
Plasmid#162844PurposeYeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #4_1DepositorInsert4_1_RNA-device
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFBP-4_1m.mutant
Plasmid#162869PurposeYeast expression of the mutant #4_1m for the RNA-based sensor of fructose-1,6-bisphosphate #4_1DepositorInsert4_1m_RNA-device
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGY416
Plasmid#166660PurposeFor light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeastDepositorInsertLiCre
UseCre/LoxTagsExpressionYeastMutationCre: E340A, D341APromoterGAL1Available sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain
Plasmid#58725PurposeExpresses GFP-Tagged CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
UseTagsGFP and MycExpressionYeastMutationPromoterPHO5Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only