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Plasmid#122089PurposeU6 driven SpCas9 sgRNA expression vector for cloning own guidesDepositorInsertSpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET19b+H2B(T116C)
Plasmid#157808PurposeExpression of human histone H2B with T116C mutation to facilitate maleimide chemistryDepositorInsertH2B(T116C)
UseTagsExpressionBacterialMutationT116CPromoterT7Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-tRNA2.0
Plasmid#158395PurposeGolden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-EF1a/b-actin
Plasmid#82583Purpose5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expressionDepositorInsertEF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-tRNA2.0
Plasmid#158396PurposeGolden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5E-smyhc1
Plasmid#126748PurposeZebrafish slow myosin heavy chain promoter. Clone 667DepositorInsertsmyhc1 (smyhc1 Zebrafish)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-NICD1
Plasmid#46048DepositorInsertNotch1 (C-term) (Notch1 Mouse)
UseEntry vectorTagsMyc tagExpressionMutationCytoplasmic domain initiating at amino acid 1753PromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-Gal4-vp16
Plasmid#171075PurposeExpresses Gal4-vp16 fusion protein in bacteriaDepositorInsertGal4 DBD (NEWENTRY Budding Yeast)
UseTagsHis and VP16 AD (413-490)ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
plk1 T210D
Plasmid#132964PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorInsertpolo-like kinase 1 (PLK1 Human)
UseTagsExpressionMammalianMutationT210DPromoterAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5E_unc503
Plasmid#64020Purpose5' Gateway entry vector with muscle-specific promoter of zebrafish unc-45bDepositorInsertmuscle-specific promoter of zebrafish unc-45b (unc45b Zebrafish)
UseGatewayTagsExpressionMutationPromoter503-bp promoter of zebrafish unc-45bAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
NmCas9_sgRNA_expression_in_pBluescript
Plasmid#122091PurposeU6 driven NmCas9 sgRNA expression vector for cloning own guidesDepositorInsertNmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFP.R377
Plasmid#138247PurposeExpresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC0x_gRNA_Pos5_Ptet
Plasmid#179336PurposegRNA Position Vector 5, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 camDepositorInsertPtet gRNA with sfGFP dropout
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSW610 - GR-LhG4_BD
Plasmid#115992Purposeentry vector for GreenGate cloning method, CDS, with adaptors for B and D modulesDepositorInsertGR-LhG4_BD
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
placZα-tdMCP
Plasmid#191132PurposeExpression of tdMCP from a lacZα inducible promoterDepositorInsertdMCP_1
UseSynthetic BiologyTagsExpressionMutationPromoterlac-inducible promoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW181 - GR-LhG4
Plasmid#115983Purposeentry vector for GreenGate cloning method, CDSDepositorInsertGR-LhG4
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ152
Plasmid#69303PurposeGateway entry vector with pco-dCas9-VP64DepositorInsertpco-dCas9-VP64 (Plant codon-optimized)
UseCRISPRTagsExpressionMutationVP64 activator fusion; NLS fusionPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only