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Plasmid#209341PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mCherry fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only
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S3_001_026_MV2
Plasmid#134422PurposepYTKMV2-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1DepositorInsertsmCherry
NLS_FdeR-N
UseTagsnoneExpressionYeastMutationnonePromoterpGMP1 and pTDH3Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-55:DMD-mEGFP
Plasmid#159744PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassetteDepositorInsertDMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cry2L348F(1-531)-mCh-BAXS184E
Plasmid#117239PurposeLight activated recruitment of BAX in mammalian cells with Cry2/CIB optogenetic switchDepositorInsertCry2531.L348F-mCh-BAXS184E
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)
Plasmid#192123PurposeLentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)DepositorInsertpEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-TdTomato-WPRE-pA
Plasmid#37092PurposeExpresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)DepositorInsertTdTomato
UseAAV and Cre/Lox; Cre-offTagsExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FKBP-Rab5-Blasticidin
Plasmid#120715PurposeExpresses early endosome-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertRab5a
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationAmino acids 2-215PromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate (c+iii)
Plasmid#192122PurposeLentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(c+iii)DepositorInsertpEF1a-anti-CD19scFV_CD28_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)
Plasmid#192124PurposepHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)DepositorInsertpEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-BiNLS-fYap
Plasmid#176623Purposeexpresses optofYap in zebrafishDepositorInsertYes associated protein (yap1 Zebrafish)
UseZebrafish expressionTagsmCherryExpressionMutationPromoterSP6Available sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA
Plasmid#37755PurposeCre-dependent expression of ChETA-tdTomato, for optogenetic excitationDepositorInsertChETA-TdTomato
UseAAV and Cre/Lox; Cre-onTagsTdTomatoExpressionMutationPromoterEF1aAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
sgRNA3_GAL4UAS-Luciferase reporter
Plasmid#64159PurposePhotoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA3 for GAL4UAS-Luciferase reporter
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-Slow-FT
Plasmid#31918DepositorInsertSlow-FT
UseTagsHisExpressionBacterialMutationM18V/E30V/K69R/L84W/A179V compared to mCherry (bu…PromoteraraBAvailable sinceSept. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFT1.0
Plasmid#46138DepositorInsertmCherry
UseCre/Lox; Mutagenic gene trap for zebrafishTagsExpressionMutationPromoterAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQP-2473
Plasmid#138976PurposeNucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.DepositorInsertNcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD
Plasmid#197687Purposeplasmid for expressing the DNMT3bCDDepositorInsertH2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Synthetic, Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCggRO-reporter
Plasmid#124582PurposeReporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)DepositorInsertCggR promoter element
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Myo1b
Plasmid#187366PurposeExpresses rat myosin 1b isoform b domain labelled with CherryDepositorInsertmyosin 1b isoform b (Myo1b Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CRY2-mCh-superPLDx10
Plasmid#188986PurposeA lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)DepositorInsertPLD
UseTagsCRY2 and mCherryExpressionMammalianMutationF163L, K249R, G328S, G381V, G429D, D480GPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGen2.1-redCALWY-4
Plasmid#50738PurposeExpresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.DepositorInsertgene for redCALWY-4 Zn2+ sensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only