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Plasmid#100736PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP2B1 (AP2B1 Human)
UseTagsPDZb and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-MQ1(C141S)-EGFP
Plasmid#89635PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG, 6*His, and T2A-EGFPExpressionMammalianMutationC141S, TGC-->TCCPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
R
Plasmid#167170PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cellsDepositorInsertsATP1A1-R
ATP1B1
UseTagsExpressionBacterial and InsectMutationPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSG5-HA-ERR3 S57,81,219A
Plasmid#37850DepositorInsertEstrogen-related receptor gamma (Esrrg Mouse)
UseTagsHAExpressionMammalianMutationchanged Serines 57, 81, and 219 to Alanine (AGC t…PromoterSV40Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJFA274
Plasmid#49239PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
UseTALEN; Tale-tet1 expression vectorTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)
Plasmid#140533PurposeOsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2DepositorInsertmAID-EGFP-NLS TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJZC620
Plasmid#62282PurposeExpresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cellsDepositorInsertsMCP-VP64
PCP-VP64
dCas9
UseTagsVP64ExpressionYeastMutationNuclease activity has been inactivated by mutatio…PromoterpAdh and pTdh3Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro
Plasmid#110830PurposeExpresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.DepositorInsertAZGP1 (Azgp1 Mouse)
UseNon-viral gene expression vectorTagsT2A and dTomatoExpressionMutationPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)
Plasmid#140535PurposeOsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2DepositorInsertmAID-EGFP-NLS TOL2
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHM053Env
Plasmid#139019PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
SunTagCACTA1g2-22aa-TET1cd
Plasmid#106437PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoterDepositorInsertCACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-3xHA-V5-RpL10Ab
Plasmid#125223Purpose3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expressionDepositorInsertRpL10Ab (RpL10Ab Fly)
UseTags3x HA, V5ExpressionInsectMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184739Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
UseTagsExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_His-ZZ-TEV-Lis1-SNAPf
Plasmid#133073PurposePlasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tagDepositorInsertLis1 (PAFAH1B1 Human)
UseTags8x His, Linker-TEV-linker_TEV, SNAPf, and ZZExpressionInsectMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV dCas9-MC (eGFP)
Plasmid#89931PurposeExpresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.DepositorInsertdCas9-MC
UseTagsFlagExpressionMammalianMutationdeactivated Cas9, fragment of M.SssI (residues 27…PromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJFA345D4
Plasmid#49238PurposeTET1-catalytic domain (CD) expression vector for TALE-TET1 fusion protein to induce targeted demethylation in mammalian cellsDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
UseTALEN; Tale-tet1 expression vectorTagsExpressionMutationPromoterEF1aAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-MCL1S159A
Plasmid#21606DepositorInsertMCL-1 S159A (MCL1 Human)
UseTagsExpressionMammalianMutationSerine 159 mutated to Alanine, abolishing phosph…PromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI
Plasmid#184740Purposeencodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109DepositorInsertFsRT-Cas1(opt)-Cas2(opt)
UseTagsExpressionBacterialMutationsequence codon optimized for expression in E. coliPromoterpTetAAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/L28F/G51PEKN
Plasmid#118585PurposeBacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationN23 mutated to P23, L28 mutated to F28, G51 mutat…PromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
S
Plasmid#167172PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cellsDepositorInsertsATP1A1-S
ATP1B1
UseTagsExpressionBacterial and InsectMutationPromoterP10 and PHAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only