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pcDNA4.1-Myc-RNF8-R42A

Plasmid

#221677

Purpose

Mammalian expression of Myc-tagged human RNF8 (R42A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Tags

Expression

Mammalian

Mutation

R42A

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-RNF8

Plasmid

#221683

Purpose

Mammalian expression of Myc-tagged BirA*-RNF8 fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSD139A

Plasmid

#218129

Purpose

Hybrid circuit displaying hsCRBN-W384A,Y386A bait (P22 anchor) and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
luxAB

Use

Tags

scP22

Expression

Mutation

W384A, Y386A

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1-3

Plasmid

#218798

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1-3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-BI-hTFR1-4

Plasmid

#218799

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1-4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AtSLAC1 8D

Plasmid

#212948

Purpose

Vector used for expression of AtSLAC1 8D in mammalian cell

Depositor

Sangho Lee

Article

Lee et al Nat Commun. 2023 Nov 14;14(1):7345. doi:

Insert

Slow anion channel-associated 1 (OZS1 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

S59D/T62D/S65D/S86D/S107D/S124D/S146D/S152D

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSN687

Plasmid

#177363

Purpose

Expresses human KIF1A(1-393)(P305L) fused with leucine zipper and His tag

Depositor

Shinsuke Niwa

Article

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Use

Tags

His tag and Leucine zipper

Expression

Bacterial

Mutation

changed Proline 305 to Leucine

Promoter

T7

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pS97splitRBP_S1dC2

Plasmid

#183769

Purpose

Expresses SHIP1-delta-C2-Enzyme domain flanked by RBP

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP1deltaC2 (INPP5D Human)

Use

Tags

6HIS, RBP (C-terminal) w/HRV3C cleavage site, and…

Expression

Bacterial

Mutation

SHIP1deltaC2 includes aa N393 to aa Q730 only

Promoter

T7lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 FDX1-1

Plasmid

#184476

Purpose

Lentivirus gRNA targeting human FDX1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIPT1-2

Plasmid

#184481

Purpose

Lentivirus gRNA targeting human LIPT1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIPT1 (LIPT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIPT1-1

Plasmid

#184480

Purpose

Lentivirus gRNA targeting human LIPT1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIPT1 (LIPT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIPT2-2

Plasmid

#184483

Purpose

Lentivirus gRNA targeting human LIPT2 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIPT2 (LIPT2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 FDX1-2

Plasmid

#184477

Purpose

Lentivirus gRNA targeting human FDX1 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIPT2-1

Plasmid

#184482

Purpose

Lentivirus gRNA targeting human LIPT2 gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIPT2 (LIPT2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pS97splitRBP_S1-Enz

Plasmid

#183767

Purpose

Expresses SHIP1-Enzyme domain flanked by RBP

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP1 (INPP5D Human)

Use

Tags

6HIS, RBP (C-terminal) w/HRV3C cleavage site, and…

Expression

Bacterial

Mutation

includes aa N393 to aa L864 only

Promoter

T7lac

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CBSH3+ 3m shRNA-1

Plasmid

#160067

Purpose

3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Tags

Expression

Mutation

GGcTGaTTaCAACAAGGATTG (mutations shown in lower c…

Promoter

mouse U6 promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- 3m shRNA3

Plasmid

#160213

Purpose

Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Tags

Expression

Mutation

TGTATGACCTCaGGcTGtACCA (mutations shown in lower …

Promoter

mouse U6 promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- 3m shRNA2

Plasmid

#160211

Purpose

Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Tags

Expression

Mutation

TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …

Promoter

mouse U6 promoter

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- 3m shRNA1

Plasmid

#160209

Purpose

Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Tags

Expression

Mutation

GATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…

Promoter

mouse U6 promoter

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(DEdel)-IDR-mCherry-CRY2-NLS

Plasmid

#145273

Purpose

Mammalian Expression of Nuclear Opto HOXD13(DEdel) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(D/E del)-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

D/E deleted

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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