Addgene

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xyl8.2

Plasmid

#107216

Purpose

Xylanase design 8.2

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

xyl8.2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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xyl8.3

Plasmid

#107217

Purpose

Xylanase design 8.3

Depositor

Sarel Fleishman

Article

Lapidoth et al Nat Commun. 2018 Jul 17;9(1):2780. doi:

Insert

xyl8.3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pKER-Clover

Plasmid

#85974

Purpose

Expresses Clover from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

Clover

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef-1a

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomEmerald-CaURA3

Plasmid

#44890

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mEmerald with CaUra3 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomEmerald

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoMaxGFP-CaURA3

Plasmid

#44875

Purpose

Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with CaUra3 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoMaxGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoMaxGFP-SpHis5

Plasmid

#44840

Purpose

Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoMaxGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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piHGpd/G170D

Plasmid

#1328

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

GPD promoter-hsp82 ts mutant (G170D) (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

G170D

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pLENTI_hACE2_HygR

Plasmid

#155296

Purpose

Lentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistance

Depositor

Raffaele De Francesco

Article

Notarbartolo et al Sci Immunol. 2021 Aug 10;6(62). pii: 6/6

Insert

hACE2 (ACE2 Human)

Use

Lentiviral

Tags

C9

Expression

Mutation

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-FRT/TO-3xFLAG-REV7

Plasmid

#114127

Purpose

Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

REV7 (MAD2L2 Human)

Use

Genomic integration, tetracycline inducible

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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delta PE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52380

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted -1620 - -1011 upstream from OCT4 ATG

Promoter

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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delta DE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52381

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted -3194 - -1768 upstream from OCT4 ATG

Promoter

OCT4

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-FHA-SHLD2-m1

Plasmid

#114122

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (m1 mutant) (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

RNF8 FHA domain (aa 2-160) and eGFP

Expression

Mammalian

Mutation

W489A, F494A, W495A mutations introduced

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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mouse deltaPE Oct4-GFP transgenic reporter

Plasmid

#52382

Purpose

BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP pA
Oct4 (Pou5f1 Mouse)
PGK-gb2-Neo

Use

Tags

Expression

Mammalian

Mutation

deleted -300 - -1300bp fragment upstream from Oct…

Promoter

Oct4 and PGK+gb2

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta2-EGFP

Plasmid

#160979

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta2 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGGG-Sr43

Plasmid

#186974

Purpose

Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).

Depositor

Brande Wulff

Article

Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:

Insert

Sr43

Use

Tags

None

Expression

Bacterial and Plant

Mutation

None

Promoter

Natice

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP hsp70

Plasmid

#15215

Depositor

Lois Greene

Article

Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

Insert

hsp70 (HSPA1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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LentiV_Cas9_Blast

Plasmid

#125592

Purpose

Lentiviral expression of spCas9 with blasticidin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-FLPo

Plasmid

#118027

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-DIO-FLPo

Use

AAV

Tags

Expression

Mutation

Promoter

TRE

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PPP1CB-miRFP703

Plasmid

#178524

Purpose

Expresses PP1c-miRFP703 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

PP1c-miRFP703 (PPP1CB Human, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pET14b-PITPalpha

Plasmid

#58256

Purpose

bacterial expression of human PITP alpha

Depositor

Shamshad Cockcroft

Article

Hara et al J Biol Chem. 1997 Jun 6;272(23):14908-13

Insert

PITP alpha (PITPNA Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only