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Plasmid#164477PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.DepositorInsertLAMP1 (Lamp1 Rat)
UseTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-Scramble
Plasmid#175014PurposeNegative-control construct for Akt-STOPS.DepositorInsertmCherry-Akt-Scramble
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-STOPS-NLS
Plasmid#175007PurposeNuclear-targeted Akt-STOPS.DepositorInsertmCherry-Akt-STOPS-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AktAR2-NES
Plasmid#175011PurposeCytosol-targeted AktAR2.DepositorInsertAktAR-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKH115_GALNT2-keima
Plasmid#214969PurposeGolgiphagy flux reporter GALNT2-keimaDepositorInsertGALNT2-keima (GALNT2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCosSAD1AprR
Plasmid#190786PurposeAn E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombinationDepositorInsertsupstream region of the aur1 cluster for auricin
ampramycin resistance cassette
downstream region of the aur1 cluster for auricin
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-L
Plasmid#182279PurposeMammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.DepositorInsertcAMPFIRE-L (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-Foxo1ADA (pCMV5)
Plasmid#12143DepositorInsertFoxo1 ADA (Foxo1 Mouse)
UseTagsHA and mycExpressionMammalianMutationPhosphorylation-deficient, also referred to as co…PromoterCMVAvailable sinceAug. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_cAMPFIRE-M
Plasmid#182280PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.DepositorInsertcAMPFIRE-M (cAMP sensor)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmStrawberry-Atg4BC74A
Plasmid#21076DepositorInsertAtg4B(C74A) (Atg4b Mouse)
UseTagsmStrawberryExpressionMammalianMutation220-222 TGC(Cys) is changed to GCA (Ala)PromoterAvailable sinceJune 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNAFLAG-ATM-kd
Plasmid#43908DepositorInsertataxia telangiectasia mutated (ATM Human)
UseTagsFLAG and HisExpressionMammalianMutationkinase dead (D2870A and N2875K)PromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MITF_P2A_Hygro_Barcode
Plasmid#120460PurposeBarcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMITF (MITF Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-RdLight1
Plasmid#125706PurposeExpresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.DepositorInsertRdLight1
UseTagsFlag tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF1A
Plasmid#172206PurposeExpresses KIF1A tagged with eGFP in mammalian cellsDepositorInsertKIF1A (Kif1a Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B tdTomato
Plasmid#116870PurposeNuclear localized tdTomatoDepositorInsertH2B tdTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
SaCas9 TadDE
Plasmid#193846PurposeExpress TadDE (with SaCas9) in mammalian cellsDepositorInsertTadDE with SaCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Abl-His6-FLAG
Plasmid#52684Purposeexpression of Abl in mammalian cellsDepositorInsertABL1 (Abl1 Mouse)
UseTagsFLAG and His6ExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
retro-gRNA-eGFP
Plasmid#116926PurposeRetrovirus expressing eGFP with BBSI cloning sites for sgRNADepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterEF1aAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2B-GFP
Plasmid#116869PurposeNuclear localized GFPDepositorInsertGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
12x PRE TK luc
Plasmid#206163PurposeLuciferase reporter construct containing 12 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350DepositorInsert12X PRE TK luciferase
UseLuciferaseTagsExpressionMammalianMutationPromotermin TKAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only