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Plasmid#111834PurposeFor mamalian expression of Ecdysone receptor-based inducible transgene expression switch.DepositorInsertGAL4-minimal VP16-EcR (EcR Synthetic)
UseTagsExpressionMammalianMutationPromoterCMV, SV40, 10XUASAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE_HRASV12_Hyg
Plasmid#195143PurposeHRas G12V overexpression vector with hygroymycin resistanceDepositorInserthRAS (HRAS Human)
UseTagsExpressionMammalianMutationG12VPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216249PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminusDepositorInsertCas9 and sgRANGAP.C1 (RANGAP1 Human)
UseCRISPR; Endogenous taggingTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso C
Plasmid#137721PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 short isoformDepositorInsertBRD4 short isoform (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA
Plasmid#107270PurposeExpresses Cas9 upon induction with doxicycline.DepositorInsertshSpCas9
rtTA
bGH polyA-SV40polyA
UseTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationPromoterCAG and TREAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-HNF4A
Plasmid#219396PurposeExpress HNF4A in mammalian cellsDepositorInsertHNF4A (HNF4A Human)
UseTagsN terminal Myc tagExpressionMammalianMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hGPR56-IRES-GFP
Plasmid#52297PurposeExpresses human GPR56 and GFP in mammalian cells.DepositorInsertHuman GPR56 coding sequence (ADGRG1 Human)
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHes1(467 RBPj(-))-luc
Plasmid#43805DepositorInsertHes1 Promoter (-467 to +46) RBPj(-) (Hes1 Mouse)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationContains the murine Hes1 promoter (-467 to +46), …PromoterHes1 promoter fragment (-467 to +46) (RBPj-)Available sinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Gata4
Plasmid#72690PurposeDoxycyline regulated expression of Gata4 in mammalian cellsDepositorInsertGata 4 (Gata4 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13(+10A)-IDR-mCherry
Plasmid#145282PurposeBacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion ProteinDepositorInsertHOXD13(+10A)-IDR (HOXD13 Human)
UseTagsExpressionBacterialMutationA-repeat expandedPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep
Plasmid#176534PurposeBacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrepDepositorInsertCRISPR-associated IscB (Delaware Bay metagenome)
UseTags14xHis-MBP-bdSUMO and TwinStrepExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf6(WT)-mCherry
Plasmid#79422PurposeExpresses C-terminally mCherry-tagged Arf6(WT) in mammalian cellsDepositorInsertArf6 (ARF6 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SMS1-TEV-Twin-Strep
Plasmid#202528PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cellDepositorInsertSGMS1 (SGMS1 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)PromoterCAG and chicken β-actin promoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3/flag-pbira(1-g78)-sec61b
Plasmid#154455Purposeexpresses flag-pbira(1-g78)-sec61b/biochemical assayDepositorInsertflag-pbira(1-g78)-sec61b (SEC61B Human)
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9pUbi-N
Plasmid#106331PurposeExpression of Cas9 in plants, ZmUbi promoter, Kanamycin selectionDepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…PromoterZmUbiAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-Ago2
Plasmid#72207PurposeMammalian expression of full-length human Ago2DepositorInsertargonaute RISC catalytic component 2 (AGO2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13-IDR-mCherry-CRY2-NLS
Plasmid#145260PurposeMammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLSDepositorInsertHOXD13wt-IDR (HOXA13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/hArf1(WT)-mCherry
Plasmid#79419PurposeExpresses C-terminally mCherry-tagged Arf1(WT) in mammalian cellsDepositorInsertArf1 (ARF1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pHR-MED1trunc-IDR-YFP-NLS
Plasmid#145275PurposeMammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 NLSDepositorInsertMED1-IDR truncated (MED1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only