-
Plasmid#178057PurposeExpression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.DepositorInsertXRI-FLAG
UseAAVTagsFLAG-MBPExpressionMammalianMutationPromoterHuman ubiquitin C (UBC) promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2NS-IRES-dOrai-IRES-GFP
Plasmid#72895PurposeMammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFPDepositorInsertdmBACCS2NS-IRES-dOrai-IRES-GFP
UseTagsIRES-GFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)
Plasmid#197885PurposeCan be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInsertGFP
UseAAVTagsExpressionMutationN/APromoterCAGAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-DNAseI
Plasmid#182409PurposeBacterial expression of Vesicle Nucleating peptide-DNaseI fusionDepositorInsertVNp-DNase I (DNASE1 Bovine)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)
Plasmid#197893PurposeCan be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInsertmCherry
UseAAVTagsExpressionMutationN/APromoterEF1aAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
StrKDEL-IRES-ManII-mScarlet-i
Plasmid#117274PurposeExpresses an Str-KDEL Hook for the RUSH system and separately mannosidase II-mScarlet-iDepositorInsertsUseTagsmScarlet-iExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMV and IRESAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-Akt1-K179M
Plasmid#73409PurposeExpresses kinase-inactive mutant of Akt with HA tagDepositorInsertAKT1 (AKT1 Human)
UseTagsHA tagExpressionMammalianMutationchanged K179M to make Kinase inactive mutantPromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hSTING (N154S)
Plasmid#214160PurposeTransient expression of gain-of-function STING mutantDepositorInsertSTING (N154S) (STING1 Human)
UseTagsExpressionMammalianMutationN154SPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CIB-VP16 (B1014)
Plasmid#92036PurposeExpresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.DepositorInsertCIB1 (CIB1 Mustard Weed)
UseTagsFusion of plant CIB1 (intact NLS) with VP16 activ…ExpressionMammalianMutationPromoterT7Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-AtAFB2-mCherry
Plasmid#129716PurposeAAVS1 safe harbor targeting vector expressing AtAFB2-mCherryDepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherryExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hSTING (V155M)
Plasmid#214161PurposeTransient expression of gain-of-function STING mutantDepositorInsertSTING (V155M) (STING1 Human)
UseTagsExpressionMammalianMutationV155MPromoterCMVAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGM190
Plasmid#69614Purposeinducible gene expression in StreptomycesDepositorInsertsaphII
tsr
rep
sso
TipA promoter
dso
UseStreptomyces- e. coli shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2-IRES-dOrai-IRES-mCherry
Plasmid#72894PurposeMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherryDepositorInsertdmBACCS2-IRES-dOrai-IRES-mCherry
UseTagsIRES-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-Akt1-aa1-149
Plasmid#73410PurposeExpresses HA tagged aa1-149 of AKT1DepositorInsertAKT1 (AKT1 Human)
UseTagsHA tagExpressionMammalianMutationExpresses aa 1-149 only of AKT1PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-Akt1-aa120-433
Plasmid#73411PurposeExpresses HA tagged aa120-433 AKT1DepositorInsertAKT1 (AKT1 Human)
UseTagsHA tagExpressionMammalianMutationExpresses aa 120-433 of AKT1PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe HA-∆Raf1(S642A):ER* puro
Plasmid#72572PurposeExpresses hormone-responsive, feedback-resistant Raf allele with an N-terminal HA tag.DepositorInsertRaf1 (RAF1 Human)
UseRetroviralTagsER* and HAExpressionMammalianMutationamino acids 306-648, serine 642 mutated to alaninePromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3Basic-miR126-EGFL7-Promoter
Plasmid#32244DepositorUseLuciferaseTagsExpressionMammalianMutationPromoter region 5' UTRPromoter5' UTRAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
PH-Btk(R28C)-GFP
Plasmid#51464PurposeNon-PIP3 binding control for PH-Btk-GFPDepositorInsertBTK (aa 1–177) (BTK Human)
UseTagsGFPExpressionMammalianMutationR28CPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA-Akt1-aa1-408
Plasmid#73412PurposeExpresses HA tagged aa1-408 of AKT1DepositorInsertAKT1 (AKT1 Human)
UseTagsHA tagExpressionMammalianMutationExpresses aa 1-408 of AKT1PromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYL214
Plasmid#171031PurposeCRISPR plasmids encodes Cas9 and a sgRNA targeting EZH2 exon 2 - intron 2 junction (sequence: GCAGACGAGCTGATGAAGTAA)DepositorInsertEZH2 (EZH2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only