Addgene

We narrowed to 11,785 results for: CAR

Showing: 5401 - 5420 of 11785 results

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pcDNA3 Barr2 flag

Plasmid

#14685

Depositor

Robert Lefkowitz

Article

Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

Insert

beta-arrestin 2 (Arrb2 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
μ2-HA-D176A

Plasmid

#32754

Depositor

Alexander Sorkin

Article

Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

Insert

μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

D176A

Promoter

CMV

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pET3a-OSF-CypA

Plasmid

#79039

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OneSTrEP-FLAG- Cyclophilin A (PPIA Human)

Use

Tags

OneSTrEP-FLAG

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre stuffer v3

Plasmid

#158030

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pREDTAI

Plasmid

#51627

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-His-TEV-mCherry-p62

Plasmid

#199780

Purpose

Used for the expression of mCherry labelled p62 (SMC Internal No. 391).

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Use

Tags

6x His, TEV, mCherry

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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RNA motif plasmid cloning backbone

Plasmid

#107253

Purpose

RNA motif plasmid with BoxB sites. Digest with BsmBI and insert RNA motif of interest. Stuffer size 1.8kb

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

RNA motif plasmid cloning backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(human)

Plasmid

#79034

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human) (TRIM5 Human)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID_pAS31

Plasmid

#118220

Purpose

expresses 3xHA-tagged TurboID in C. elegans intestine

Depositor

Jessica Feldman

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Tags

3x HA tag

Expression

Worm

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

ges-1p

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pREDIA

Plasmid

#51625

Purpose

E. coli genome modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Tags

Expression

Bacterial

Mutation

Promoter

pRha

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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I48 ELP

Plasmid

#68937

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of Isoleucine guest residue

Depositor

Andrew MacKay

Article

Shi et al J Control Release. 2013 Nov 10;171(3):33

Insert

I48 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pWaldo-GFPe_PepTSo

Plasmid

#58334

Purpose

Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

Depositor

Simon Newstead, So Iwata

Article

Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

Insert

PepTso

Use

Tags

GFP-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pILGFP91

Plasmid

#83548

Purpose

Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

PGK1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Cav1-mRFP

Plasmid

#14434

Depositor

Ari Helenius

Article

Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

Insert

Caveolin-1 (CAV1 Canis familalis)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Claspin

Plasmid

#12659

Depositor

Michele Pagano

Article

Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

Insert

Claspin (CLSPN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2007

Availability

Academic Institutions and Nonprofits only
pAPM-miR30-DDX58-ts3

Plasmid

#174243

Purpose

DDX58 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX58 shRNA (RIGI Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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aav-hsp68-mCherry-MCS-pA

Plasmid

#195412

Purpose

AAV9 based MPRA assay

Depositor

William Pu

Article

Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pKP112 (pRS406-GFP-ADH1t)

Plasmid

#106472

Purpose

Vector for creating C-terminal GFP fusion proteins in yeast.

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Type

Empty backbone

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

None

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-1

Plasmid

#92156

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP670nano3

Plasmid

#184666

Purpose

Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-human alpha-tubulin

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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