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Plasmid#102930PurposeExpress a lysosomal TagDepositorInsertTmem192 (TMEM192 Human)
UseLentiviralTagsHAExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNEU-hMbPylRS-4xU6M15
Plasmid#105830PurposePlasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cellsDepositorInsertsHumanized MbPylRS
tRNAM15
UseTagsExpressionMammalianMutation(Y271A, Y349F)MbPylRSPromoterCMV and U6Available sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-EKAR4
Plasmid#205513PurposeGenetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.DepositorInsertendo-EKAR4
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSGV-1D3-28Z All ITAMs intact
Plasmid#107226PurposeAnti-mouse CD19 CAR with CD3 ITAMs intactDepositorInsert1D3-28Z All ITAMs intact
UseRetroviralTagsNoneExpressionMutationNonePromoterAvailable sinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLenti sfGFP-LAMP1-mCherry (pHLARE)
Plasmid#164478PurposeLysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.DepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsmCherry and superfolder GFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-Ubiquitin
Plasmid#18712PurposeMammalian expression of human ubiquitin with HA tagDepositorInsertubiquitin (UBB Human)
UseTagsHAExpressionMammalianMutationThe HA-Ubiquitin insert is from the UBB gene. The…PromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSV human p85 alpha (HA tag)
Plasmid#11499DepositorInsertp85 alpha (PIK3R1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462) V2.0
Plasmid#62987PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaCTD_P2A_Hygro_Barcode
Plasmid#120511PurposeBarcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaCTD (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of carboxy-terminal domainPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaNTD_P2A_Hygro_Barcode
Plasmid#120510PurposeBarcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaNTD (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of amino-terminal domainPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltab_P2A_Hygro_Barcode
Plasmid#120507PurposeBarcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltab (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of basic motifPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-Tmem192-2xFlag
Plasmid#102929PurposeExpress a lysosomal TagDepositorInsertTmem192 (TMEM192 Human)
UseLentiviralTagsFlagExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-MAT2A
Plasmid#100521PurposeDox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)DepositorInsertMAT2A (MAT2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterpTight TREAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL1142 (pSPIN, pBBR1 backbone)
Plasmid#160730PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER Long P2A
Plasmid#164107PurposeDetect the long Mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP Mt-ER Long P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPLICS Mt-ER Short P2A
Plasmid#164108PurposeDetect the short Mitochondria-Endoplasmic reticulum contactDepositorInsertSplit GFP Mt-ER Short P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1
Plasmid#85069PurposeYellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)DepositorInsertPH-PLCD1 (PLCD1 Human)
UseTagsIRES-sYFP2-PH and mScarletExpressionMammalianMutationPLCD aa 1–170PromoterCMVAvailable sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_H2A_C1
Plasmid#85051PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorInsertH2A (H2AC16 Human)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TC1154
Plasmid#164876PurposeSplit GFP reporter plasmid expressing GFP (b11betaDepositorInsertSplit GFP(beta11)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only