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pBABE puro mouse IRS-1 myc

Plasmid

#11374

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-1 (Irs1 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE-Flag-Cdk9-IRES-eGFP

Plasmid

#28096

Depositor

Andrew Rice

Article

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
HA-HIF2alpha-P405A/P531A-pBabe-puro

Plasmid

#19006

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: P405A and P531A

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-2 myc

Plasmid

#11373

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-HIF2α P405A/P531A-pBabe-Hygro

Plasmid

#21676

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

cDNA changed amino acids from wt: Proline 405 t…

Promoter

Available since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-HIF1alpha P402A/P564A-pBabe-puro

Plasmid

#19005

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-Puro-Myr-Flag-AKT1

Plasmid

#15294

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Tags

Myr-Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-puro-Halo-hMRE11(H129N)

Plasmid

#208394

Purpose

To generate stable cell line expressing Halo-human MRE11(H129N) by Retroviral infection.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human MRE11 (MRE11 Human)

Use

Luciferase and Retroviral

Tags

Expression

Mammalian

Mutation

H129N

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-2XFLAG,2XHA-CyclinF-puro

Plasmid

#32976

Depositor

Michele Pagano

Article

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
SS-3xHA-TurboID-KDEL pBabe-puro

Plasmid

#186011

Purpose

Expresses TurboID confined to ER by human albumin signal sequence (SS) and KDEL. 3xHA tag.

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SS-3xHA-TurboID-KDEL

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1271 pBabe puroL Myr HA Akt2

Plasmid

#9018

Depositor

William Sellers

Insert

Akt2 (AKT2 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
1272 pBabe puroL Myr HA Akt3

Plasmid

#9019

Depositor

William Sellers

Insert

Akt3 (AKT3 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pBabe-Puro-RhoA FLARE.sc Biosensor

Plasmid

#12602

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-Flag-IDH1-R132H

Plasmid

#62924

Purpose

Retrovirus expression plasmid

Depositor

Yue Xiong

Article

Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

Insert

IDH1 (IDH1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

mutation Arg132His

Promoter

Available since

April 6, 2016

Availability

Academic Institutions and Nonprofits only
pBabe-puro GSK3 beta (S9A)

Plasmid

#14128

Depositor

Brendan Manning

Article

Zhang et al Mol Cell. 2006 Oct 20. 24(2):185-97.

Insert

GSK3 beta (S9A) (GSK3B Human)

Use

Retroviral

Tags

V5 tag

Expression

Mammalian

Mutation

S9A. Mutant version of GSK3 that lacks the inhibi…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pBabe puro H-Ras G12V T35S

Plasmid

#12274

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V T35S (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Contains G12V activating mutation. The T35S mutat…

Promoter

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pBabe 3XFLAG-wtGATA6-3XAU1 puro

Plasmid

#72607

Purpose

Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
1119 pBabe puroL HA FKHR AAA

Plasmid

#9025

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

FKHR AAA (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

T24A S256A S319A

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
E-1x-BioID2 pBabe-puro (SARS-CoV-2)

Plasmid

#185987

Purpose

Expresses SARS-CoV-2 Envelope protein fused to BioID2 and HA tag

Depositor

Kyle Roux

Article

May et al Viruses. 2022 Mar 15;14(3). pii: v140306

Insert

SARS-CoV-2 Envelope protein (E SARS-CoV-2)

Use

Retroviral

Tags

BioID2 and HA

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE puro human RXR alpha

Plasmid

#11441

Depositor

Ronald Kahn

Insert

RXR alpha (RXRA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only