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Plasmid#105136PurposeYeast gene targetingDepositorInsertkanMX6-P3nmt1-mCFP
UseTags3nmt1-mCFPExpressionBacterial and YeastMutationA206KPromoter3nmt1Available sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
mGDF8 donor CFP-hDF
Plasmid#72590PurposePlasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.DepositorInsertdysferlin (DYSF Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
hGDF8 donor CFP-hDF
Plasmid#72591PurposePlasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.DepositorInsertdysferlin (DYSF Human)
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
CFP(1-158) in pcDNAI/Amp
Plasmid#60895PurposeThis vector attaches CFP(1-158) to the N-terminus of a protein.DepositorInsertCFP(1-158)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-CFP
Plasmid#50482Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChr-alpha6 (Chrna6 Mouse)
UseTagsCFP fusion in M3-M4 loop (after residue A405)ExpressionMammalianMutationGly-Ala-Gly flexible linker flanking the CFP open…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CMV-CFP;UBC-Cre25nt
Plasmid#65727PurposeExpresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.DepositorInsertsCMV promoter driving CFP expression
UBC promoter driving Cre-25nt expression
UseCre/LoxTagsExpressionMammalianMutationPromoterCMV and UBCAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 884-1066-ECFP
Plasmid#46182DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsECFP and HisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CFP-SaCas9-HF (without sgRNA)
Plasmid#134470PurposeHuman expression vector for SaCas9-HF variant.DepositorInsertStaphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
UseCRISPRTagsSV40NLS-3xFLAGExpressionMammalianMutationR245A, N413A, N419A, R654APromoterCAGAvailable sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lyn-FKBP-FKBP-CFP (LF2C)
Plasmid#20149DepositorInsertLyn-FKBP-FKBP-CFP (FKBP1A Human)
UseTagsExpressionMammalianMutationSee depositor comments below.PromoterAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
MSCV--Mouse Mdm2 wt-IRES-CFP
Plasmid#180493PurposeRole of Mdm2 splicing variants in tumorigenesisDepositorInsertMdm2 full length (wt)
UseRetroviralTagsExpressionMammalianMutationPromoterMSCVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
FH1-PNGT#1-mCherry_PGK-H2B-CFP
Plasmid#164103PurposeFluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.DepositorInsertPNGT#1-mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
FH1-MGT#1-mVenus_PGK-H2B-CFP
Plasmid#164100PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#1-mVenus
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
FH1-MGT#2-mCherry_PGK-H2B-CFP
Plasmid#164102PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#2-mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
ECFP-Bcr in pCCL-cppt178-MNDU3
Plasmid#38156DepositorInsertBcr (BCR Human)
UseLentiviralTagsECFPExpressionMutationPromoterAvailable sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-GAP43-ECFP-stop-L2
Plasmid#186356PurposeEntry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequencesDepositorInsertGrowth-associated protein-43 (Gap43 Mouse, Synthetic)
UseExpression of a fluorescent membrane markerTagsECFPExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Zeo-ecfp
Plasmid#65038PurposeZeoR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-CFP-STM2(15-746)
Plasmid#18863DepositorInsertStromal Interaction Molecule 2 (STIM2 Human)
UseTagsCFPExpressionMammalianMutationCFP inserted between signal peptide and residues …PromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)
Plasmid#55199PurposeCFP reporter along with FF4 miRNA binding sites and 28 nt sequencesDepositorInsertECFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRetro-XT-PAK2-CFP(puro)-T402A
Plasmid#81179PurposeRetroviral vector for expression of kinase inactive PAK2 with a CFP fusionDepositorInsertp21 (RAC1) activated kinase 2 (PAK2 Human)
UseRetroviralTagsCFPExpressionMutationT402APromoterP-tight TREAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TNT-CFP-STIM1-C227W
Plasmid#68403PurposeExpresses a STIM1 gain-of-function mutant in mammalian cellsDepositorInsertCFP-STIM1 (STIM1 Human)
UseTagsCyan Fluorescent Protein (CFP)ExpressionMammalianMutationChanged Cysteine 227 to Tryptophan; CFP was inser…PromoterCMVAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only