-
Plasmid#207505PurposeThis plasmid can be used to generate retrovirus.DepositorInsertBATF, HA-GD2-28z_CAR (BATF Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p6187
Plasmid#198158Purposebacterial expression of sifA::HaloTag fusion, mutant formDepositorInsertPsifA sifA delta331-336::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
UseTagsHaloTag-HAExpressionBacterialMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-P4Mx1
Plasmid#108121PurposePI4P biosensorDepositorInsertX.leavis map2k1.L(32-44):EGFP:L. penomophila SidM(546-647)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-ROC2
Plasmid#19899DepositorInsertRING finger protein 2 (ROC2) (RNF7 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC19A3
Plasmid#132045PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC19A3 (SLC19A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-iLACCO1
Plasmid#218821PurposeMammalian expression of genetically encoded green fluorescent L-lactate biosensor with mild affinityDepositorInsertiLACCO1
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMLS7-egfp
Plasmid#112917PurposeFluorescence microscopyDepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPS7 promoter and Ps7Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCWori-A13AMO-aaCPRct
Plasmid#20117DepositorInsertAmorphadiene oxidase and p450 reductase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRIPK1-Flag
Plasmid#112487PurposeMammalian expression of Flag-hRIPK1. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE131
Plasmid#118485Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHRi_1G4 hTCRβ-SNAP
Plasmid#187604PurposeLentiviral expression of hTCR beta(1G4), and SNAP for low expressionDepositorInsert1G4 hTCR beta, and SNAP tag (TRB Human)
UseLentiviralTagsExpressionMammalianMutationPromotermHSPAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoSNAP
Plasmid#136623PurposePlasmid encoding outer mitochondrial localization SNAP-TagDepositorInsertNTOM20-SNAP-Tag
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP
Plasmid#188901PurposedCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening elementDepositorInsertSID-dCas9-XTEN80-KRAB(Kox1)
UseLentiviralTagsHA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…ExpressionMutationPromoterSFFVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC19A1
Plasmid#132266PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC19A1 (SLC19A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pExp-sfGFP
Plasmid#112573PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-sfGFP and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cdk1-mCherry
Plasmid#54466PurposeCDK1-mCherry in pIVT vector for in vitro transcriptionDepositorInsertCdk1 mCherry
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV.MRE11i
Plasmid#15565DepositorInsertMRE11 shRNA (MRE11 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTW195b63
Plasmid#136931PurposeDirected evolution of SpCas11DepositorInsertgIII(N)-npuN
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p32Lb
Plasmid#158154PurposeConstruct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMIND
Plasmid#24730DepositorInsertTetRO promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pC4-RhE-FRB-Fis1
Plasmid#68056Purposeexpress Fis1 tail-anchored FRB for heterodimerization with FKBP in mammalian cellsDepositorInsertFis1 (FIS1 Human)
UseTagsFRBExpressionMammalianMutationFis1 tail (aa 93-152)PromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB90 - pAGM4723_VirGN54D
Plasmid#123187PurposeGolden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in AgrobacteriaDepositorTypeEmpty backboneUseLevel 2 acceptor vector for expression in plantsTagsExpressionPlantMutationVirGN54D for increase virulencePromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE137
Plasmid#118491Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmClover3
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF0337
Plasmid#143395PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMECOM (MECOM Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRIPK1-Flag-delN
Plasmid#112492PurposeMammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationdeletion of N terminus (AA1-274 deleted)PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRIPK1-Flag-delC
Plasmid#112493PurposeMammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationdeletion of C terminus (AA290-671 deleted)PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-tdTomato-miRE_shRen-Puro
Plasmid#135687PurposeConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10-Claudin-1
Plasmid#46387PurposeClaudin-1 promoter reporter assayDepositorInsertCLDN1 Promoter (CLDN1 Human)
UseLuciferaseTagsExpressionMutationPromoterCLDN1 promoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZE31-DsRed-ms2x2
Plasmid#26248DepositorInsertms2 binding sites (x2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMRE133
Plasmid#118487Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His
Plasmid#171587PurposeBacterial expression of NLS-RfxCas13d-NLS 6HisDepositorInsertMKIEE-NLS-RfxCas13d-NLS-HA-6His
UseTagsHA-6HIS and MKIEEExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag AKAP8L
Plasmid#20481DepositorInsertAKAP8L (AKAP8L Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-Raichu-454HRasCT
Plasmid#176029PurposeA FRET biosensor for HRas activityDepositorInsertRaichu-Ras-HRasCT
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
p4305
Plasmid#198150Purposebacterial expression of sifA::HaloTag fusionDepositorInsertPsifA sifA::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
UseTagsHaloTag-HAExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GDI2
Plasmid#67093PurposeStandard for cell-free expression benchmarking.DepositorInsertGDI2 (GDI2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330 PLIN2
Plasmid#202196PurposepX330 backbone expressing sgRNA to edit the C-terminus of human PLIN2DepositorInsertPLIN2 (PLIN2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-UBC-M2rtTA-H2BmNeon (donorAB-UBC-mNeon)
Plasmid#85800PurposedonorAB-UBC-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BneonDepositorInsertPPP1R12C (PPP1R12C Human)
UseHuman targetingTagsExpressionMutationhomology arms for knock-in into first intronPromoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_TCF3_FLI1
Plasmid#205934PurposeExpress mEGFP-tagged fusion protein, TCF3_FLI1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRE136
Plasmid#118490Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRIPK1-Flag-delDD
Plasmid#112494PurposeMammalian expression of the indicated Flag- hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationdeletion of DD domain (AA584-671 deleted)PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only