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Plasmid#137929PurposeExpression of cjCas9 with Hif1a targeting sgRNADepositorInsertCjCas9
UseAAV and CRISPRTagsSV40NLS-HA-T2A-EGFPExpressionMutationPromoterEF-1alphaAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_IRF1
Plasmid#167760PurposeExpresses IRF1 in mammalian cellsDepositorInsertIRF1 (IRF1 Human)
UseTagsMACExpressionMammalianMutationno mutationsPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Rab21
Plasmid#83421PurposeMouse Rab21 (wild type)DepositorInsertRab21 (Rab21 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Raptor_1 shRNA
Plasmid#1857DepositorInsertRaptor (RPTOR Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV Puro DEST p38KTRmCerulean3
Plasmid#59155PurposeLentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
2HA-pDEST-C
Plasmid#118374PurposeGateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2HAExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_WT REV1
Plasmid#201586PurposeExpresses human DNA repair protein REV1 with an N-terminal HA tag in mammalian cellsDepositorInsertDNA repair protein REV1 (REV1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 human PP2A Aα full length
Plasmid#132634Purposeexpresses human PP2A Aα full length in E. coliDepositorInsertPP2A Aα (PPP2R1A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#191797Purposevector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoterDepositorInserthumanized dCas13d
UseCRISPRTagsExpressionMammalianMutationR239A, H244A, R858A, H863APromoterCBh, SV40, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9-Gem
Plasmid#80424PurposeUsed for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joiningDepositorInsertSpCas9-Gem
UseCRISPRTagsGemininExpressionMutationcodon optimized for humanPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-endo-Nb37
Plasmid#205514PurposeEndosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.DepositorInsertendo-Nb37
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP5
Plasmid#22590DepositorInsertUSP5 (USP5 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#102951PurposeExpresses FLAG-APEX2 in Saccharomyces cerevisiae under control of GAL1 promoterDepositorInsertAPEX2-NES
UseTagsFLAGExpressionYeastMutationPromoterGAL1Available sinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-8
Plasmid#72347PurposeMammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-RIPK3
Plasmid#78804PurposeTo overexpress RIPK3 in Mammalian CellsDepositorInsertRIPK3 (RIPK3 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
Raptor_2 shRNA
Plasmid#1858DepositorInsertRaptor (RPTOR Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG_Xph20-mRuby2-CCR5TC
Plasmid#135531PurposeEncodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseTagsmRuby2ExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.17_PB-STEAP3-PGK-Blast
Plasmid#205562PurposeExpress human STEAP3DepositorInsertSTEAP3 (STEAP3 Human)
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIP-AMPKa1_KD
Plasmid#79011PurposeFor mammalian expression of a kinase dead (K47R) version of AMPK alpha1.DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseTagsExpressionMammalianMutationK47RPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRC-CMV-Rev1b
Plasmid#204153PurposeLentivirus helper plasmid coding for RevDepositorInsertRev
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRGEN-CMV-CjCas9
Plasmid#89752PurposeExpression of CjCas9 in mammalian cellsDepositorInsertCas9
UseCRISPRTagsSV40-HAExpressionMammalianMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCS
Plasmid#199354PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTagBFP-P2A-ALFA-SUMOEuExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGES401
Plasmid#190198PurposeFor CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selectionDepositorInsertspCas9
UseCRISPRTags3xFlagExpressionPlantMutationplant-codon optimizedPromoterGlycine max elongation factor 1A(pM4)Available sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-scr
Plasmid#169795PurposeExpresses Cas9 and guide RNA with untarget sequence generated by scramble of the target sequence of LentiCRISPRv2-ACTB-C1..DepositorInsertScrambled guide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
ΔNp63gamma-FLAG
Plasmid#27012DepositorInsertΔNp63gamma (TP63 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
mRFP-FKBP12
Plasmid#67514PurposeFKBP12 onlyDepositorInsertFKBP12 (FKBP1A Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
LV-Sox10MCS5-GFP-EF1a-mCherry
Plasmid#115782PurposeSox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.DepositorInsertsmouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
EF1alpha promoter driven constitutive mCherry
UseLentiviralTagsEF1α promoter fused to mCherry and cfos promoter …ExpressionMutationPromoterSOX10 Multiple Species Conserved enhancer element…Available sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIRE
Plasmid#26745DepositorInsertiCre expression cassette
UseCre/Lox; Flp/frtTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP1A-WT-puro
Plasmid#156166PurposeLentiviral vector for expression of RAP1A-WT, with puromycin selectionDepositorInsertRAP1A (RAP1A Human)
UseLentiviralTagsnoneExpressionMammalianMutationwild type formPromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only