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pPRISM-Fuse-cmlc2-eGFP

Plasmid

#117788

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMTB2-Avi-Cerulean-Rpl10a

Plasmid

#79880

Purpose

Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)

Use

Tags

Avi

Expression

Bacterial

Mutation

Promoter

Beta-actin2

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;mafbbCE1-P1Egfp

Plasmid

#90155

Purpose

contains mafbb enhancer sequence, and mafbb specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

mafbbE1P1Egfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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Zebrafish-gRNA-0007

Plasmid

#42247

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

gRNA-th1 (th Zebrafish)

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only
p3E-DysF

Plasmid

#109544

Purpose

Multisite gateway vector for 3' tagging with Dysferlin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dysferlin (dysf Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Please see Depositor Comments

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Bin1b

Plasmid

#176014

Purpose

Mammalian expression of zebrafish EGFP-Bin1b. Parton lab clone HYG

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Bin1b (bin1b Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Hox D13a

Plasmid

#36467

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxa11b (hoxa11b Zebrafish)

Use

Riboprobe synthesis

Tags

Expression

Mutation

F63I, P75Q and M133I

Promoter

T7

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
TAL3178

Plasmid

#41352

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-sox2-Left (sox2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
ZE4 Wnt-8b

Plasmid

#17045

Depositor

Randall Moon

Article

Kelly et al Development. 1995 Jun. 121(6):1787-99.

Insert

wnt-8b (wnt8b Zebrafish)

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
acta 2

Plasmid

#78711

Purpose

zebrafish acta2 3-UTR–specific probe

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

acta2 (acta2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

contains bp 1,243–1,591

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
360 pCR kdrb

Plasmid

#13067

Depositor

Nathan Lawson

Article

Covassin et al Proc Natl Acad Sci U S A. 2006 Apr 25. 1

Insert

kdrb (kdr Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
Tol2-lyzC-RFP-miR-223

Plasmid

#97148

Purpose

miR-223 expression

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

miR-223

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pTone-gata2aECE-Kaede

Plasmid

#132976

Purpose

gata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Inserts

Kaede
gata2a endothelial enhancer (x6) and a carp b-actin basal promoter

Use

Unspecified; Transposon-mediated

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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TAL3126

Plasmid

#41300

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-nphs2-Left (nphs2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
p3E DN Kif5a

Plasmid

#105973

Purpose

3' element for Gateway cloning, 5 single nucleotide mutations compared to the reference sequence, but none of them change the amino acidtruncation aa416-1033(end) 1854 bp

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

Kif5a (kif5aa Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

truncation of aa 416-1033

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pfli1

Plasmid

#22413

Depositor

Nathan Lawson

Article

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

fli1 (fli1 Zebrafish)

Use

Riboprobe

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only
SI10-Brevican

Plasmid

#46300

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 . 8():76.

Insert

Brevican mouse monoclonal antibody (bcan Zebrafish)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
kank2_pJet1.2

Plasmid

#121980

Purpose

for generating kank2 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank2 (LOC571549 Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnn1a

Plasmid

#164956

Purpose

Zebrafish kcnn1a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn1a (kcnn1a Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnn1b

Plasmid

#164957

Purpose

Zebrafish kcnn1b gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn1b (kcnn1b Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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