10,100 results

1. pSmartBAC-S

   Plasmid

   #107268

   Purpose

   Broad host range bacterial artificial chromosome vector

   Depositor

   Mark Liles, Ron Godiska

   Article

   Nasrin et al J Nat Prod. 2018 Jun 22;81(6):1321-1332.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-pegRNA-puro backbone

   Plasmid

   #214085

   Purpose

   Lentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIS0

   Plasmid

   #12178

   Purpose

   3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

   Depositor

   David Bartel

   Article

   Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGE334

   Plasmid

   #153243

   Purpose

   M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 783-Rx-hU6: RfxCas13d gRNA and array cloning backbone

   Plasmid

   #228360

   Purpose

   hU6-driven expression of RfxCas13d gRNAs and arrays compatible. Contains AarI sites for guide cloning flanked by 5' DR36

   Depositor

   Michael Boettcher

   Article

   El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; Rfxcas13d grna expression …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. mVenus-pBAD

   Plasmid

   #54845

   Purpose

   Localization: Protein Expression Vector , Excitation: 515, Emission: 528

   Depositor

   Michael Davidson, Atsushi Miyawaki

   Article

   Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

   Type

   Empty backbone

   Use

   Tags

   6xHis and mVenus

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pet-MBP

   Plasmid

   #209184

   Purpose

   Expression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coli

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Type

   Empty backbone

   Use

   Tags

   HisX6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE GFP

   Plasmid

   #10668

   Purpose

   Empty backbone for retroviral gene expression. Select recipient cells using GFP.

   Depositor

   William Hahn

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. L4440

   Plasmid

   #1654

   Depositor

   Andrew Fire

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   RNAi

   Tags

   T7p, T7p, lacZN, OriF1>>, OriF1<<

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPRa_all-in-one

    Plasmid

    #183695

    Purpose

    Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

    Depositor

    Edward Avezov

    Article

    Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX_HA

    Plasmid

    #178534

    Purpose

    Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

    Depositor

    William Hahn

    Article

    Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC64420

    Plasmid

    #209517

    Purpose

    Cloning in complete guide expression cassettes, with Cas9

    Depositor

    Wendy Harwood, Cristobal Uauy

    Article

    Lawrenson et al Research Square, 12 March 2024, PREPRINT

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. CROPseq-Guide-Puro-mCherry2

    Plasmid

    #219679

    Purpose

    CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

    Depositor

    Christoph Bock

    Article

    Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMH-HA

    Plasmid

    #101767

    Purpose

    Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBtet-Pur

    Plasmid

    #60507

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TCE & RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBbi-GP

    Plasmid

    #60511

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and puromycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2Flag-pDEST-C

    Plasmid

    #118372

    Purpose

    Gateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti PGK Hygro DEST (w530-1)

    Plasmid

    #19066

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAK_Tol2_TRE_Puro

    Plasmid

    #130259

    Purpose

    Tol2 doxycycline inducible expression vector

    Depositor

    Michael Yaffe

    Type

    Empty backbone

    Use

    Tol2 transposon, doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJYDN

    Plasmid

    #162450

    Purpose

    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.

    Depositor

    Gideon Schreiber

    Article

    Zahradník et al bioRxiv Preprint 2020

    Type

    Empty backbone

    Use

    Shuttle vector bacteria/yeast

    Tags

    Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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