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PLX_307 SLC31A1

Plasmid

#184491

Purpose

Lentivirus expression for SLC31A1 gene

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

SLC31A1 (SLC31A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG DEPTOR (13×S/T->A)

Plasmid

#21702

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

DEPTOR 13xA (DEPTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Nonphosphorylatable mutant. 13xA. Also has N204S …

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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ptdTomato-N1-FLCN

Plasmid

#49174

Purpose

Mammalian expression of mouse folliculin (FLCN) fused to tdTomato

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin (Flcn Mouse)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-FNIP1

Plasmid

#49175

Purpose

Mammalian expression of mouse folliculin interacting protein 1 (FNIP1) fused to GFP

Depositor

Shawn Ferguson

Article

Petit et al J Cell Biol. 2013 Sep 30;202(7):1107-112

Insert

Folliculin Interacting Protein 1 (Fnip1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Stop codon deleted

Promoter

CMV

Available since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-Hs4E-BP1

Plasmid

#79438

Purpose

Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4E-BP1 (EIF4EBP1 Human)

Use

Tags

lambdaN-HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 1 DEPTOR

Plasmid

#21337

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

DEPTOR shRNA 1 (Deptor Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2009

Availability

Academic Institutions and Nonprofits only
pLNHA-C1-HsTTP_1-313_AH

Plasmid

#148879

Purpose

Mammalian Expression of HsTTP_1-313

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsTTP_1-313 (ZFP36 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF1_AE

Plasmid

#148569

Purpose

Mammalian Expression of HsGIGYF1

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1 (GIGYF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSV CDK9 HA (P#598)

Plasmid

#14638

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 (CDK9 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn_CapChR1_eYFP

Plasmid

#188033

Purpose

Expresses CapChR1-eYFP in mammalian neurons

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR1-eYFP

Use

AAV

Tags

eYFP

Expression

Mutation

S63D, L132C, T159C and N258E mutations in CrChR2

Promoter

hSyn

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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DsRed +9e

Plasmid

#191766

Purpose

Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

DsRed

Use

Tags

N terminal fusion of positively charged polypetod…

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO mouse shRNA 2 DEPTOR

Plasmid

#21338

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

DEPTOR shRNA 2 (Deptor Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
eSrtA-MT3

Plasmid

#200305

Purpose

Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA

Depositor

Artur Krężel

Article

Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

Insert

eSrtA-LPETG-MT3 (MT3 Human)

Use

Tags

His-tag and SUMO-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_B1ChR2_TS_mScarlet_ER

Plasmid

#195191

Purpose

Highly K+ selective channelrhodopsin

Depositor

Johannes Vierock, Peter Hegemann

Article

Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

Insert

Bilabrum sp. K+ selective channelrhodopsin 2

Use

Tags

ER-export sequence, Kir2.1 trafficking motif, and…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Hs4EBP3_36-69-L61A V67A-GB1_AB

Plasmid

#148380

Purpose

Bacterial Expression of Hs4EBP3_36-69-L61AV67A

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4EBP3_36-69-L61AV67A (EIF4EBP3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Hs4EBP3_36-69-Y40AL45A-GB1_AA

Plasmid

#148280

Purpose

Bacterial Expression of Hs4EBP3_36-69-Y40AL45A

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4EBP3_36-69-Y40AL45A (EIF4EBP3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-Hs4EBP3_36-69-YLLVtoAAA-GB1_AA

Plasmid

#148281

Purpose

Bacterial Expression of Hs4EBP3_36-69-YLLVtoAAAA

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Hs4EBP3_36-69-YLLVtoAAAA (EIF4EBP3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-F41DW42D_AB

Plasmid

#148372

Purpose

Insect Expression of Dm4E-T-F41DW42D

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dm4E-T-F41DW42D (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

5 non silent mutation L127S, E493G, H628R, N659S,…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T-YLFWtoAADD_AB

Plasmid

#148373

Purpose

Insect Expression of Dm4E-T-YLFWtoAADD

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

Dm4E-T-YLFWtoAADD (4E-T Fly)

Use

Tags

Expression

Insect

Mutation

two non silent mutations H611R and N659S and 8 si…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_32-83-GB1_AA

Plasmid

#148267

Purpose

Bacterial Expression of DmThor_32-83

Depositor

Elisa Izaurralde

Article

Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

Insert

DmThor_32-83 (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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