eSrtA-MT3
(Plasmid #200305)

• Purpose: Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
• Depositing Lab: Artur Krężel
• Publication: Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125. doi: 10.1186/s12934-023-02134-x.

Backbone

• Vector backbone: pETM11
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eSrtA-LPETG-MT3
• Species: H. sapiens (human)
• Entrez Gene: MT3 (a.k.a. GIF, GIFB, GRIF, ZnMT3)
• Promoter: T7
• Tag / Fusion Protein:
  • His-tag and SUMO-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry