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Plasmid#87002Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertbarcode landing site-mCherry-4xboxB
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pERB161
Plasmid#58286Purposeconst. Mis12-GFP-FKBP3; ind. FRB-mCH-Mad1^485-584 (MID)DepositorInsertsMis12-GFP-FKBPx3
inducible FRB-mCh-Mad1^485-584
FKBP-targeted miRNA
UseTagsGFP and mCherryExpressionMammalianMutationamino acids 485-584 of Mad1PromoterAvailable sinceFeb. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSX2719
Plasmid#200763PurposeExpresses mCherry fused MS2 coat protein and 24xGCN4 (Suntag) with the tissue-specific promoter semo-1 in C. elegans.DepositorInsertMCP-24xGCN4
UseTagsExpressionWormMutationPromotersemo-1Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCS108
Plasmid#209329PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mCherry fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
RGG-BcLOV-mCh
Plasmid#208276PurposeExpresses RGG-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.DepositorInsertRGG-BcLOV-mCh
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fus-BcLOV-mCh
Plasmid#208277PurposeExpresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.DepositorInsertFus-BcLOV-mCh
UseTagsExpressionMammalianMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
XLone-GvpB
Plasmid#173798PurposeMammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)DepositorInsertGvpB
UseTagsmCherry (K187N -please see depositor comments)ExpressionMammalianMutationPromoterTRE3G promoterAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOxyR_rfp
Plasmid#194155PurposeRed Fluorescent Reporter of intracellular hydrogen peroxideDepositorInserttranscriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry
UseTagsExpressionBacterialMutationPromoterPoxyR, PoxySAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLBC0140
Plasmid#172794PurposeLevel 0 module containing CDS2ns_mCHERRYDepositorInsertCDS mCHERRY
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBAR-Cry2PHR-WH2
Plasmid#176605PurposeCry2PHR with N-terminal IBAR domain and C-terminal WH2 domainDepositorInsertIBAR-Cry2PHR-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cry2PHR-IBAR-WH2
Plasmid#176603PurposeCry2PHR with C-terminal IBAR and WH2 domainsDepositorInsertCry2PHR-mCherry-IBAR-WH2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZS212
Plasmid#177639PurposeIn vitro transcription of Patiria miniata Lamp1DepositorInsertLamp1
UseIn vitro transcriptionTagsmCherryExpressionMutationPromoterAvailable sinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZS218
Plasmid#177641PurposeIn vitro transcription of Patiria miniata Rab7DepositorInsertRab7
UseIn vitro transcriptionTagsmCherryExpressionMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)
Plasmid#165026PurposeExpress cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFPDepositorInsertLaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)
Plasmid#165028PurposeExpress cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFPDepositorInsertLaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFP.R373
Plasmid#138243PurposeExpresses the split mCherry reporter interrupted by the SaP-dpol intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R362
Plasmid#138232PurposeExpresses the split mCherry reporter interrupted by the CbP-RNR artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R376
Plasmid#138246PurposeExpresses the split mCherry reporter interrupted by the Pfu-RIR1-1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQP-iRISB
Plasmid#138979PurposeLight-controlled tri-directional targeting of endogenous proteinsDepositorInsertNcoI-BphP1-CAAX-IRES2-NES-SGlinkeriB( GFP)-mCherry-Q-PAS1-AsLOV2cNLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 539
Plasmid#138078PurposeControl vector with no degradation tag to compare against various degradation tag variantsDepositorInsertJ23104-mCherry::sbfI- pSEVA-331Bb
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only