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ADAMTS5_pCSdest

Plasmid

#53819

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ADAMTS5 (ADAMTS5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pSFFV-SpyTag-sfCherry2(11)-TagBFP

Plasmid

#117485

Purpose

Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyTag002-GG-sfCherry2(11)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF3370

Plasmid

#144846

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYBL1 (MYBL1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hOKMS

Plasmid

#51543

Purpose

Inducible expression of polycistronic 2A spaced OCT4-KLF4-MYC-SOX2

Depositor

Tarjei Mikkelsen

Article

Cacchiarelli et al Cell. 2015 Jul 16;162(2):412-24. doi: 10

Insert

hOKMS (MYC, KLF4, SOX2, POU5F1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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AiP978-pAAV-mscRE4-minBGpromoter-FlpO-WPRE-hGHpA

Plasmid

#163472

Purpose

Direct-expressing FlpO AAV Virus. Alias: AiP978 - pAAV-AiE2004m-minBG-FlpO-WPRE-HGHpA

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

FlpO

Use

AAV

Tags

Expression

Mutation

Promoter

Beta Globin minimal promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-mRuby2

Plasmid

#175294

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mRuby2

Use

Tags

mRuby2

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti CMV V5-LUC Blast (w567-1)

Plasmid

#21474

Purpose

3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, Blast

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

V5-LUC

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pSBtet-Hyg

Plasmid

#60508

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and hygromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

TCE & RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-H1.5

Plasmid

#32898

Depositor

Michael Hendzel

Article

Th'ng et al J Biol Chem. 2005 Jul 29;280(30):27809-1

Insert

Histone H1.5 (H1-5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2018

Availability

Academic Institutions and Nonprofits only
FUW-tetO-loxP-hNANOG

Plasmid

#60849

Purpose

Lentiviral DOX-inducible expression vector for human NANOG

Depositor

Rudolf Jaenisch

Article

Theunissen et al Cell Stem Cell. 2014 Oct 2;15(4):471-87.

Insert

hNANOG (NANOG Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCIN BRCA2 WT

Plasmid

#16245

Depositor

Mien-Chie Hung

Article

Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

Insert

BRCA2 (BRCA2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pHAGE-TGFB1

Plasmid

#116799

Purpose

Lentiviral expression of TGFB1

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFB1 (TGFB1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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ISDra2-TnpB-mCherry-U6-ωRNA*

Plasmid

#212967

Purpose

Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter

Depositor

Chunlong Xu

Article

Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

Inserts

ISDra2-TnpB-T2A-mCherry
ωRNA*

Use

CRISPR

Tags

NLS and NLS-FLAG

Expression

Mammalian

Mutation

Promoter

CAG, T7 and U6

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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RA-Sec61β

Plasmid

#153978

Purpose

Dimerization dependent fluorescent protein RA domain fused to ER protein Sec61β

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

Sec61 Transolocon Subunit Beta (SEC61B Human)

Use

Tags

pAcGFP-C1

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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TurboID-EV

Plasmid

#215085

Purpose

Expresses TurboID in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

TurboID (birA )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

Plasmid

#186993

Purpose

AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122, non-targeting crRNA guide

Use

AAV

Tags

Expression

Mutation

s1006-gggs-d1221 deletion

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-MKLP1

Plasmid

#70145

Purpose

mammalian expression of MKLP1 with an eGFP fusion

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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PresentER-Cassette GFP

Plasmid

#102942

Purpose

PresentER with removable cassette for cloning of MHC-I antigen (GFP version)

Depositor

David Scheinberg

Article

Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

Plasmid

#221225

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al bioRxiv 2024.02.01.577593

Insert

AID-PcrA M6-UGI-T2A-mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR8-YFP

Plasmid

#13024

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 8 (TLR8 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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