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Plasmid#87174PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterConstitutive proDAvailable sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)
Plasmid#86922PurposeTo express Ubl4A (1-89 residues) tagged with FLAG-EGFP at N- terminus.DepositorInsertUBL4A (UBL4A Human)
UseTagsFLAG and GFPExpressionMammalianMutationResidues from 90-157 is deletedPromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala11
Plasmid#87172PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterConstitutive proAAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala12
Plasmid#87173PurposeNADPH dependent Alanine Dehydrogenase, Constitutive E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterConstitutive proCAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
const #7-RFP
Plasmid#81081Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23109Available sinceAug. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pconst #6-RFP
Plasmid#81080Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23117Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWHI2-HYG
Plasmid#80589PurposeCEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoterDepositorInsertWHI2 (WHI2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterWHI2 native promoterAvailable sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24_BD3179
Plasmid#73142PurposeExpresses in E.coli B. bacteriovorus Bd3179DepositorInsertBd3179
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRFP shRNA-resistant TrkB F616A -S478A
Plasmid#72718PurposeMammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant and lacking TIAM1-binding siteDepositorInsertTrkB
UseTagsRFPExpressionMammalianMutationshRNA-resistant TrkB (T300C, T301A, C302G, C306A,…PromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR SS287AA
Plasmid#72703PurposeLentiviral expression of GR Phospho-deficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationSS287AAPromoterubiquitinAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
MS4A1 RFP (805)
Plasmid#62009Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertMS4A1
UseTagsmRFPExpressionMammalianMutationPromoterpgkAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
NET31 mRFP-N2 (490)
Plasmid#61984Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET31 (TMEM209 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-SBL1(160E,HisTagDel)
Plasmid#66879PurposeExpresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removedDepositorInsertsoybean lipoxygenase-1 (S160E)
UseTagsExpressionBacterialMutationS160EPromoterT7lacAvailable sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Xl-VSP1 R152Q
Plasmid#51881Purposeexpression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cellsDepositorInsertVSP1 R152Q (tpte2 Frog)
UseXenopus oocyte expressionTagsExpressionMammalianMutationR152Q: near the extracellular side of the fourth …PromoterCMVAvailable sinceMay 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
ptCMV-136/63-VR-HD
Plasmid#50699PurposeFinal destination vectorDepositorInsertTALEN-FokI nuclease backbone
UseTALENTagsFlagExpressionMutationPromoterCMVAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
ptCMV-136/63-VR-NG
Plasmid#50700PurposeFinal destination vectorDepositorInsertTALEN-FokI nuclease backbone
UseTALENTagsFlagExpressionMutationPromoterCMVAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
ptCMV-136/63-VR-NI
Plasmid#50701PurposeFinal destination vectorDepositorInsertTALEN-FokI nuclease backbone
UseTALENTagsFlagExpressionMutationPromoterCMVAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
ptCMV-136/63-VR-NN
Plasmid#50702PurposeFinal destination vectorDepositorInsertTALEN-FokI nuclease backbone
UseTALENTagsFlagExpressionMutationPromoterCMVAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
ptCMV-153/47-VR-HD
Plasmid#50703PurposeFinal destination vectorDepositorInsertTALEN-FokI nuclease backbone
UseTALENTagsFlagExpressionMutationPromoterCMVAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
ptCMV-153/47-VR-NI
Plasmid#50705PurposeFinal destination vectorDepositorInsertTALEN-FokI nuclease backbone
UseTALENTagsFlagExpressionMutationPromoterCMVAvailable sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only