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pCAGGS-SMS2-TEV-Twin-Strep

Plasmid

#202529

Purpose

Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Insert

SGMS2 (SGMS2 Human)

Use

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Mutation

deleted stop codon (TGA)

Promoter

CAG and chicken β-actin promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shFDPS #2

Plasmid

#198760

Purpose

conditional knockdown of FDPS

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

Insert

shFDPS #2 (FDPS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shFDPS #1

Plasmid

#198759

Purpose

conditional knockdown of FDPS

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

Insert

shFDPS #1 (FDPS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-Blasticidin-hOCT4 S236D

Plasmid

#198765

Purpose

expression of hOCT4 mutants

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

hOCT4 236D (POU5F1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S236D

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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Chr1q_Cassette-Proximal_gRNA

Plasmid

#195127

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controls

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

Plasmid

#190154

Purpose

Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp3 (Vamp2 Rat)

Use

AAV

Tags

P2AT2A-EGFP-caax

Expression

Mammalian

Mutation

Truncation that includes only rat Vamp3 AA #1-81

Promoter

MBP

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5-STABLE2-neo-RvCAHS3

Plasmid

#192472

Purpose

Stable expression of RvCAHS3 (fly codon optimized) in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3

Use

Tags

T2A-EGFP-T2A-neoR

Expression

Insect

Mutation

Promoter

Ac5 promoter

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pS97splitRBP_S2-Enz

Plasmid

#183768

Purpose

Expresses SHIP2-Enzyme domain flanked by RBP

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP2 (INPPL1 Human)

Use

Tags

6HIS, RBP (C-terminal) HRV3C cleavage site, and R…

Expression

Bacterial

Mutation

includes aa K414 to aa V872 only

Promoter

T7lac

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CBSH3- shRNA2

Plasmid

#160210

Purpose

Knock Down MTLSH3- Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat, Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- shRNA3

Plasmid

#160212

Purpose

Knock Down MYDSH3- Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat, Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- shRNA1

Plasmid

#160208

Purpose

Knock Down MQWSH3- Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat, Human)

Use

RNAi

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(+7A)-IDR-YFP-NLS

Plasmid

#145278

Purpose

Mammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+7A)-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeat expanded

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXA13(+7A)-IDR-mCherry

Plasmid

#145285

Purpose

Bacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13(+7A)-IDR (HOXA13 Human)

Use

Tags

Expression

Bacterial

Mutation

A-repeat expanded

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXA13(WT)-IDR-mCherry

Plasmid

#145284

Purpose

Bacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13(WT)-IDR (HOXA13 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXD13(+7A)-IDR-mCherry

Plasmid

#145281

Purpose

Bacterial Expression of 6xHis HOXD13 (+7A) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+7A)-IDR (HOXD13 Human)

Use

Tags

Expression

Bacterial

Mutation

A-repeat expanded

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD11-Adel-IDR

Plasmid

#145241

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXD11-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD11-Adel-IDR (HOXD11 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeats deleted

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13+7A-IDR

Plasmid

#145239

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13+7A-IDR (HOXA13 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeat expanded

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HEY2wt-IDR

Plasmid

#145235

Purpose

Expresses fusion of GAL4 DNA-binding domain and HEY2wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HEY2wt-IDR (HEY2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-GSX2wt-IDR

Plasmid

#145233

Purpose

Expresses fusion of GAL4 DNA-binding domain and GSX2wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

GSX2wt-IDR (GSX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291A

Plasmid

#159160

Purpose

Expresses DEPTOR S286/287/291A mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to alanine

Promoter

CMV

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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