Showing: 5321 - 5340 of 16015 results
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Plasmid#212575PurposeLentiviral expression of Flag-tagged mouse Miwi in mammalian cellsDepositorInsertMiwi (Piwil1 Mouse)
UseLentiviralTags5x Flag and SNAP tagExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-Flag-Mili-EGFP
Plasmid#212576PurposeLentiviral expression of Flag-tagged mouse Mili in mammalian cellsDepositorInsertMili (Piwil2 Mouse)
UseLentiviralTags5x Flag and SNAP tagExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_NIPBL_mEGFP_mAID
Plasmid#217664PurposeRepair template to endogenously tag NIPBL with mEGFP-mAID TagDepositorInsertNIPBL_mEGFP_mAID (NIPBL Human)
UseTagsmAID and meGFPExpressionBacterial and MammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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pCMV6M-Mst2
Plasmid#84296PurposeExpresses Myc-tagged Mst2DepositorInsertMst2 (STK3 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pMA3200
Plasmid#46855PurposeA lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY and myc-tagged destabilized FKBP12DepositorInsertExoY
UseLentiviralTagsFKBP E31G, R71G, K105E and MycExpressionMutationPromoterTRE-TightAvailabilityAcademic Institutions and Nonprofits only -
pMA3228
Plasmid#46856PurposeA lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12DepositorInsertExoY K81M
UseLentiviralTagsFKBP E31G, R71G, K105E and MycExpressionMutationPromoterTRE-TightAvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-SpCas9
Plasmid#104588PurposeAAV vector expressing Myc-tagged SpCas9 under the EFS promoterDepositorInsertStreptococcus pyogenes Cas9
UseAAV and CRISPRTagsMycExpressionMammalianMutationPromoterEFSAvailabilityAcademic Institutions and Nonprofits only -
pSUMO2
Plasmid#52259PurposeBacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailabilityAcademic Institutions and Nonprofits only -
pSUMO3
Plasmid#52260PurposeBacterial expression of human 6His-tagged SUMO3, SUMO-E1 and SUMO-E2DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH-8his (Merlin)
Plasmid#136447PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tagDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsShort GGSG linker and 8his tagExpressionMammalianMutationExtracellular domain (a.a. 1-716). Codon-optimize…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-8his (Merlin)
Plasmid#136454PurposeMammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tagDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsLinker and 8his tag (GGSGHHHHHHHH, includes BamHI…ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES
Plasmid#138299PurposeEncodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKCsub(T/A)-RLuc8N-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pT2/CMV-eGFP.PB/PTK
Plasmid#170540PurposeExpresses the puromycin resistance gene fused to a thymidine kinase, upon excision of this cassette the reading frame of eGFP is restoredDepositorInsertDelta 5'-eGFP CMV-PuroTK- Delta 3'-eGFP
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPBT/EFS-Cas9-PuroTk
Plasmid#170542PurposeExpresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinaseDepositorInsertPuroTK
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-DCX-TurboID-V5
Plasmid#190740PurposeMammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)DepositorInsertdoublecortin (DCX Human)
UseTagsTurboID and V5ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
His-DNPH1
Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertDNPH1 (DNPH1 Human)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pEC3115 (pSpy0K6-P23_mKate2~*ssrA)
Plasmid#218509Purposeintegrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombinationDepositorInsertmKate2
UseTagsssrA degradation tagExpressionBacterialMutationDeletion of Plac_mrfp cassette, introduction of a…PromoterP23AvailabilityAcademic Institutions and Nonprofits only -
pE-mApple L5L2
Plasmid#187690PurposeC-Term Gateway cloning tagDepositorInsertmApple
UseGateway entry vectorTagsmAppleExpressionMutationWTPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-neo-cterminal-3HA
Plasmid#102643PurposeTo generate three copies of the HA epitope tag on cDNAs.DepositorTypeEmpty backboneUseTags3x HA (YPYDVPDYA)ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 5321 - 5340 of 16015 results